Magnetogenetic Control of Protein Gradients Inside Living Cells with High Spatial and Temporal Resolution
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Etoc, Fred | |
dc.contributor.author | Vicario, Chiara | |
dc.contributor.author | Lisse, Domenik | |
dc.contributor.author | Siaugue, Jean-Michel | |
dc.contributor.author | Piehler, Jacob | |
dc.contributor.author | Coppey, Mathieu | |
dc.contributor.author | Dahan, Maxime | |
dc.date.accessioned | 2021-12-23T16:20:16Z | - |
dc.date.available | 2021-12-23T16:20:16Z | - |
dc.date.issued | 2015 | |
dc.identifier.issn | 15306984 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/13386 | - |
dc.description.abstract | Tools for controlling the spatial organization of proteins are a major prerequisite for deciphering mechanisms governing the dynamic architecture of living cells. Here, we have developed a generic approach for inducing and maintaining protein gradients inside living cells by meaty of biofunctionalized magnetic nanoparticles (MNPs). For this purpose; we tailored the size and surface properties of MNPs in order to ensure unhindered mobility in the cytosol. These MNPs with a core diameter below 50 nm could be rapidly relocalized in living cells by exploiting biased diffusion at weak magnetic forces in the femto-Newton range. In combination with MNP surface functionalization for specific in situ capturing of target proteins as well as efficient delivery into the cytosplasm, we here present a comprehensive technology for controlling intracellular protein gradients with a temporal resolution of a few tens of seconds. | |
dc.description.sponsorship | French National Research Agency (ANR) Paris-Science-Lettres ProgramFrench National Research Agency (ANR) [ANR-10-IDEX-0001-02 PSL]; Labex CelTisPhyBio [ANR-10-LBX-0038]; Human Frontier Science ProgramHuman Frontier Science Program [RGP0005/2007]; M.D. acknowledges financial support from French National Research Agency (ANR) Paris-Science-Lettres Program (ANR-10-IDEX-0001-02 PSL), Labex CelTisPhyBio (No. ANR-10-LBX-0038), the Human Frontier Science Program (Grant RGP0005/2007). | |
dc.language.iso | en | |
dc.publisher | AMER CHEMICAL SOC | |
dc.relation.ispartof | NANO LETTERS | |
dc.subject | cell signaling | |
dc.subject | Chemistry | |
dc.subject | Chemistry, Multidisciplinary | |
dc.subject | Chemistry, Physical | |
dc.subject | intracellular diffusion | |
dc.subject | LIGHT | |
dc.subject | LIVE CELLS | |
dc.subject | MACROMOLECULES | |
dc.subject | MAGNETIC NANOPARTICLES | |
dc.subject | Magnetogenetics | |
dc.subject | Materials Science | |
dc.subject | Materials Science, Multidisciplinary | |
dc.subject | MOLECULES | |
dc.subject | nanomagnetism | |
dc.subject | nanoparticles | |
dc.subject | Nanoscience & Nanotechnology | |
dc.subject | Physics | |
dc.subject | Physics, Applied | |
dc.subject | Physics, Condensed Matter | |
dc.subject | protein manipulation | |
dc.subject | Science & Technology - Other Topics | |
dc.subject | SPATIOTEMPORAL CONTROL | |
dc.subject | SYSTEMS | |
dc.subject | VELOCITY | |
dc.title | Magnetogenetic Control of Protein Gradients Inside Living Cells with High Spatial and Temporal Resolution | |
dc.type | journal article | |
dc.identifier.doi | 10.1021/acs.nanolett.5b00851 | |
dc.identifier.isi | ISI:000354906000106 | |
dc.description.volume | 15 | |
dc.description.issue | 5 | |
dc.description.startpage | 3487 | |
dc.description.endpage | 3494 | |
dc.contributor.orcid | 0000-0003-1217-9493 | |
dc.contributor.orcid | 0000-0001-8924-3233 | |
dc.contributor.orcid | 0000-0002-4943-2491 | |
dc.contributor.researcherid | AAA-3313-2021 | |
dc.contributor.researcherid | AAR-3207-2020 | |
dc.identifier.eissn | 15306992 | |
dc.publisher.place | 1155 16TH ST, NW, WASHINGTON, DC 20036 USA | |
dcterms.isPartOf.abbreviation | Nano Lett. | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-2143-2270 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | PiJa938 | - |
Seitenaufrufe
6
Letzte Woche
0
0
Letzter Monat
0
0
geprüft am 13.05.2024