Characterization of an archaeal photoreceptor/transducer complex from Natronomonas pharaonis assembled within styrene-maleic acid lipid particles
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Voskoboynikova, N. | |
dc.contributor.author | Mosslehy, W. | |
dc.contributor.author | Colbasevici, A. | |
dc.contributor.author | Ismagulova, T. T. | |
dc.contributor.author | Bagrov, D. V. | |
dc.contributor.author | Akovantseva, A. A. | |
dc.contributor.author | Timashev, P. S. | |
dc.contributor.author | Mulkidjanian, A. Y. | |
dc.contributor.author | Bagratashvili, V. N. | |
dc.contributor.author | Shaitan, K. V. | |
dc.contributor.author | Kirpichnikov, M. P. | |
dc.contributor.author | Steinhoff, H. -J. | |
dc.date.accessioned | 2021-12-23T16:20:17Z | - |
dc.date.available | 2021-12-23T16:20:17Z | - |
dc.date.issued | 2017 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/13399 | - |
dc.description.abstract | The styrene-maleic acid (SMA) copolymers enable a detergent-free extraction of membrane proteins from lipid bilayers yielding stable water-soluble nanocontainers with lipid-encased proteins. Here, we demonstrate the ability of the SMA copolymer to extract a complex of interacting membrane proteins from proteoliposomes, namely the archaeal sensory rhodopsin II from Natronomonas pharaonis, NpSRII, in complex with its transducer, NpHtrII. The NpSRII/NpHtrII complexes retained their structural integrity within SMA lipid particles as revealed by diverse biophysical techniques. Pulse electron paramagnetic resonance data showed the SMA-encased NpSRII/NpHtrII complexes as native-like 2 : 2 dimers with a distance between NpSRII sites 159 according to the ``V''-shaped conformation found in a crystal structure. | |
dc.description.sponsorship | German Research Foundation (DFG)German Research Foundation (DFG) [STE640/14]; Federal Ministry of Education and Research of Germany (BMBF)Federal Ministry of Education & Research (BMBF) [05K14MPA]; German Academic Exchange Service (DAAD)Deutscher Akademischer Austausch Dienst (DAAD); Russian Science FoundationRussian Science Foundation (RSF) [14-50-00029]; Russian Foundation for Basic Research (RFBR)Russian Foundation for Basic Research (RFBR) [15-54-12385, 16-02-00248]; This work was supported by German Research Foundation (DFG, STE640/14), the Federal Ministry of Education and Research of Germany (BMBF, 05K14MPA), the German Academic Exchange Service (DAAD) and the grants from the Russian Science Foundation (14-50-00029 (K. S.)) and the Russian Foundation for Basic Research (RFBR) (Project No. 15-54-12385 (K. S.), 16-02-00248 (P. T.)). We thank J. P. Klare for helpful discussions. | |
dc.language.iso | en | |
dc.publisher | ROYAL SOC CHEMISTRY | |
dc.relation.ispartof | RSC ADVANCES | |
dc.subject | Chemistry | |
dc.subject | Chemistry, Multidisciplinary | |
dc.subject | DETERGENT-FREE ISOLATION | |
dc.subject | DISTANCE MEASUREMENTS | |
dc.subject | ELECTRON-PARAMAGNETIC-RESONANCE | |
dc.subject | MEMBRANE-PROTEIN | |
dc.subject | MICROBIAL RHODOPSINS | |
dc.subject | MOLECULE HEIGHT MEASUREMENTS | |
dc.subject | PROTEIN-COUPLED RECEPTORS | |
dc.subject | SENSORY-RHODOPSIN-II | |
dc.subject | SIGNAL-TRANSDUCTION | |
dc.subject | VIBRATIONAL ANALYSIS | |
dc.title | Characterization of an archaeal photoreceptor/transducer complex from Natronomonas pharaonis assembled within styrene-maleic acid lipid particles | |
dc.type | journal article | |
dc.identifier.doi | 10.1039/c7ra10756k | |
dc.identifier.isi | ISI:000414780700030 | |
dc.description.volume | 7 | |
dc.description.issue | 81 | |
dc.description.startpage | 51324 | |
dc.description.endpage | 51334 | |
dc.contributor.orcid | 0000-0002-6355-7282 | |
dc.contributor.orcid | 0000-0002-5888-0157 | |
dc.contributor.orcid | 0000-0001-7773-2435 | |
dc.contributor.orcid | 0000-0001-8170-1607 | |
dc.contributor.orcid | 0000-0001-5844-3064 | |
dc.contributor.researcherid | AAH-3608-2021 | |
dc.contributor.researcherid | E-5867-2014 | |
dc.contributor.researcherid | H-3791-2014 | |
dc.contributor.researcherid | B-2375-2014 | |
dc.contributor.researcherid | J-8086-2013 | |
dc.identifier.eissn | 20462069 | |
dc.publisher.place | THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND | |
dcterms.isPartOf.abbreviation | RSC Adv. | |
dcterms.oaStatus | Green Submitted, gold | |
crisitem.author.dept | FB 04 - Physik | - |
crisitem.author.dept | Sonderforschungsbereich 944: Physiologie und Dynamik zellulärer Mikrokompartimente | - |
crisitem.author.deptid | fb04 | - |
crisitem.author.deptid | organisation19 | - |
crisitem.author.orcid | 0000-0003-2317-0144 | - |
crisitem.author.orcid | 0000-0002-8129-3081 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | VoNa568 | - |
crisitem.author.netid | MoWa274 | - |
crisitem.author.netid | IsTT001 | - |
crisitem.author.netid | KiMP001 | - |
Seitenaufrufe
4
Letzte Woche
0
0
Letzter Monat
0
0
geprüft am 29.05.2024