Characterization of an archaeal photoreceptor/transducer complex from Natronomonas pharaonis assembled within styrene-maleic acid lipid particles

DC ElementWertSprache
dc.contributor.authorVoskoboynikova, N.
dc.contributor.authorMosslehy, W.
dc.contributor.authorColbasevici, A.
dc.contributor.authorIsmagulova, T. T.
dc.contributor.authorBagrov, D. V.
dc.contributor.authorAkovantseva, A. A.
dc.contributor.authorTimashev, P. S.
dc.contributor.authorMulkidjanian, A. Y.
dc.contributor.authorBagratashvili, V. N.
dc.contributor.authorShaitan, K. V.
dc.contributor.authorKirpichnikov, M. P.
dc.contributor.authorSteinhoff, H. -J.
dc.date.accessioned2021-12-23T16:20:17Z-
dc.date.available2021-12-23T16:20:17Z-
dc.date.issued2017
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/13399-
dc.description.abstractThe styrene-maleic acid (SMA) copolymers enable a detergent-free extraction of membrane proteins from lipid bilayers yielding stable water-soluble nanocontainers with lipid-encased proteins. Here, we demonstrate the ability of the SMA copolymer to extract a complex of interacting membrane proteins from proteoliposomes, namely the archaeal sensory rhodopsin II from Natronomonas pharaonis, NpSRII, in complex with its transducer, NpHtrII. The NpSRII/NpHtrII complexes retained their structural integrity within SMA lipid particles as revealed by diverse biophysical techniques. Pulse electron paramagnetic resonance data showed the SMA-encased NpSRII/NpHtrII complexes as native-like 2 : 2 dimers with a distance between NpSRII sites 159 according to the ``V''-shaped conformation found in a crystal structure.
dc.description.sponsorshipGerman Research Foundation (DFG)German Research Foundation (DFG) [STE640/14]; Federal Ministry of Education and Research of Germany (BMBF)Federal Ministry of Education & Research (BMBF) [05K14MPA]; German Academic Exchange Service (DAAD)Deutscher Akademischer Austausch Dienst (DAAD); Russian Science FoundationRussian Science Foundation (RSF) [14-50-00029]; Russian Foundation for Basic Research (RFBR)Russian Foundation for Basic Research (RFBR) [15-54-12385, 16-02-00248]; This work was supported by German Research Foundation (DFG, STE640/14), the Federal Ministry of Education and Research of Germany (BMBF, 05K14MPA), the German Academic Exchange Service (DAAD) and the grants from the Russian Science Foundation (14-50-00029 (K. S.)) and the Russian Foundation for Basic Research (RFBR) (Project No. 15-54-12385 (K. S.), 16-02-00248 (P. T.)). We thank J. P. Klare for helpful discussions.
dc.language.isoen
dc.publisherROYAL SOC CHEMISTRY
dc.relation.ispartofRSC ADVANCES
dc.subjectChemistry
dc.subjectChemistry, Multidisciplinary
dc.subjectDETERGENT-FREE ISOLATION
dc.subjectDISTANCE MEASUREMENTS
dc.subjectELECTRON-PARAMAGNETIC-RESONANCE
dc.subjectMEMBRANE-PROTEIN
dc.subjectMICROBIAL RHODOPSINS
dc.subjectMOLECULE HEIGHT MEASUREMENTS
dc.subjectPROTEIN-COUPLED RECEPTORS
dc.subjectSENSORY-RHODOPSIN-II
dc.subjectSIGNAL-TRANSDUCTION
dc.subjectVIBRATIONAL ANALYSIS
dc.titleCharacterization of an archaeal photoreceptor/transducer complex from Natronomonas pharaonis assembled within styrene-maleic acid lipid particles
dc.typejournal article
dc.identifier.doi10.1039/c7ra10756k
dc.identifier.isiISI:000414780700030
dc.description.volume7
dc.description.issue81
dc.description.startpage51324
dc.description.endpage51334
dc.contributor.orcid0000-0002-6355-7282
dc.contributor.orcid0000-0002-5888-0157
dc.contributor.orcid0000-0001-7773-2435
dc.contributor.orcid0000-0001-8170-1607
dc.contributor.orcid0000-0001-5844-3064
dc.contributor.researcheridAAH-3608-2021
dc.contributor.researcheridE-5867-2014
dc.contributor.researcheridH-3791-2014
dc.contributor.researcheridB-2375-2014
dc.contributor.researcheridJ-8086-2013
dc.identifier.eissn20462069
dc.publisher.placeTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND
dcterms.isPartOf.abbreviationRSC Adv.
dcterms.oaStatusGreen Submitted, gold
crisitem.author.deptFB 04 - Physik-
crisitem.author.deptSonderforschungsbereich 944: Physiologie und Dynamik zellulärer Mikrokompartimente-
crisitem.author.deptidfb04-
crisitem.author.deptidorganisation19-
crisitem.author.orcid0000-0003-2317-0144-
crisitem.author.orcid0000-0002-8129-3081-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.parentorgFB 05 - Biologie/Chemie-
crisitem.author.grandparentorgUniversität Osnabrück-
crisitem.author.netidVoNa568-
crisitem.author.netidMoWa274-
crisitem.author.netidIsTT001-
crisitem.author.netidKiMP001-
Zur Kurzanzeige

Seitenaufrufe

4
Letzte Woche
0
Letzter Monat
0
geprüft am 29.05.2024

Google ScholarTM

Prüfen

Altmetric