DC Field | Value | Language |
dc.contributor.author | Kozuleva, Marina | |
dc.contributor.author | Goss, Tatjana | |
dc.contributor.author | Twachtmann, Manuel | |
dc.contributor.author | Rudi, Katherina | |
dc.contributor.author | Trapka, Jennifer | |
dc.contributor.author | Selinski, Jennifer | |
dc.contributor.author | Ivanov, Boris | |
dc.contributor.author | Garapati, Prashanth | |
dc.contributor.author | Steinhoff, Heinz-Juergen | |
dc.contributor.author | Hase, Toshiharu | |
dc.contributor.author | Scheibe, Renate | |
dc.contributor.author | Klare, Johann P. | |
dc.contributor.author | Hanke, Guy T. | |
dc.date.accessioned | 2021-12-23T16:20:18Z | - |
dc.date.available | 2021-12-23T16:20:18Z | - |
dc.date.issued | 2016 | |
dc.identifier.issn | 00320889 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/13400 | - |
dc.description.abstract | In linear photosynthetic electron transport, ferredoxin: NADP(H) oxidoreductase (FNR) transfers electrons from ferredoxin (Fd) to NADP+. Both NADPH and reduced Fd (Fd(red)) are required for reductive assimilation and light/dark activation/deactivation of enzymes. FNR is therefore a hub, connecting photosynthetic electron transport to chloroplast redox metabolism. A correlation between FNR content and tolerance to oxidative stress is well established, although the precise mechanism remains unclear. We investigated the impact of altered FNR content and localization on electron transport and superoxide radical evolution in isolated thylakoids, and probed resulting changes in redox homeostasis, expression of oxidative stress markers, and tolerance to high light in planta. Our data indicate that the ratio of Fd(red) to FNR is critical, with either too much or too little FNR potentially leading to increased superoxide production, and perception of oxidative stress at the level of gene transcription. In FNR overexpressing plants, which show more NADP(H) and glutathione pools, improved tolerance to high-light stress indicates that disturbance of chloroplast redox poise and increased free radical generation may help ``prime'' the plant and induce protective mechanisms. In fnr1 knock-outs, the NADP(H) and glutathione pools are more oxidized relative to the wild type, and the photoprotective effect is absent despite perception of oxidative stress at the level of gene transcription. | |
dc.description.sponsorship | Deutsche Forschungsgeneinschaft through Project 2 in the Collaborative Research Center at the University of Osnabruck [(SFB) 944]; [HA 5921/2-1]; This work was supported by the Deutsche Forschungsgeneinschaft through funding of Project 2 in the Collaborative Research Center (SFB) 944 at the University of Osnabruck. M.K. was supported by grant HA 5921/2-1 for the initiation of international collaboration. | |
dc.language.iso | en | |
dc.publisher | OXFORD UNIV PRESS INC | |
dc.relation.ispartof | PLANT PHYSIOLOGY | |
dc.subject | FERREDOXIN-NADP(+) REDUCTASE | |
dc.subject | MALATE-DEHYDROGENASE | |
dc.subject | NADP+ REDUCTASE | |
dc.subject | OXIDATIVE STRESS | |
dc.subject | PHOTOSYSTEM-I | |
dc.subject | Plant Sciences | |
dc.subject | PLUS OXIDOREDUCTASE | |
dc.subject | SALICYLIC-ACID | |
dc.subject | SINGLET OXYGEN | |
dc.subject | THYLAKOID MEMBRANE | |
dc.subject | WRKY70 TRANSCRIPTION FACTOR | |
dc.title | Ferredoxin:NADP(H) Oxidoreductase Abundance and Location Influences Redox Poise and Stress Tolerance | |
dc.type | journal article | |
dc.identifier.doi | 10.1104/pp.16.01084 | |
dc.identifier.isi | ISI:000391172300011 | |
dc.description.volume | 172 | |
dc.description.issue | 3 | |
dc.description.startpage | 1480 | |
dc.description.endpage | 1493 | |
dc.contributor.orcid | 0000-0001-7705-8831 | |
dc.contributor.orcid | 0000-0002-5761-5968 | |
dc.contributor.orcid | 0000-0002-5888-0157 | |
dc.contributor.orcid | 0000-0002-1247-7282 | |
dc.contributor.researcherid | AAH-7278-2020 | |
dc.contributor.researcherid | C-1428-2009 | |
dc.contributor.researcherid | H-3791-2014 | |
dc.contributor.researcherid | B-2557-2016 | |
dc.identifier.eissn | 15322548 | |
dc.publisher.place | JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA | |
dcterms.isPartOf.abbreviation | Plant Physiol. | |
dcterms.oaStatus | Bronze, Green Published, Green Submitted | |
crisitem.author.dept | FB 04 - Physik | - |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb04 | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-6140-6181 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | StHe633 | - |
crisitem.author.netid | ScRe288 | - |
crisitem.author.netid | HaGu059 | - |