Accumulation of Sulfonamide Resistance Genes in Arable Soils Due to Repeated Application of Manure Containing Sulfadiazine

Autor(en): Heuer, Holger
Solehati, Qodiah
Zimmerling, Ute
Kleineidam, Kristina
Schloter, Michael
Mueller, Tanja
Focks, Andreas 
Thiele-Bruhn, Soeren
Smalla, Kornelia
Stichwörter: AGRICULTURAL SOILS; ANTIBIOTIC-RESISTANCE; Biotechnology & Applied Microbiology; COMMUNITY STRUCTURE; ENVIRONMENT; IMPACT; INCP-1-BETA GROUP; Microbiology; PIG SLURRY; RESIDUES; SPREAD MANURE; VETERINARY MEDICINES
Erscheinungsdatum: 2011
Herausgeber: AMER SOC MICROBIOLOGY
Journal: APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volumen: 77
Ausgabe: 7
Startseite: 2527
Seitenende: 2530
Zusammenfassung: 
Two soils were amended three times with pig manure. The abundance of sulfonamide resistance genes was determined by quantitative PCR 2 months after each application. In both soils treated with sulfadiazine-containing manure, the numbers of copies of sul1 and sul2 significantly increased compared to numbers after treatments with antibiotic-free manure or a control and accumulated with repeated applications.
ISSN: 00992240
DOI: 10.1128/AEM.02577-10

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