ESEEM Reveals Bound Substrate Histidine in the ABC Transporter HisQMP(2)
DC Element | Wert | Sprache |
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dc.contributor.author | Isaev, Nikolay | |
dc.contributor.author | Heuveling, Johanna | |
dc.contributor.author | Ivanisenko, Nikita | |
dc.contributor.author | Schneider, Erwin | |
dc.contributor.author | Steinhoff, Heinz-Juergen | |
dc.date.accessioned | 2021-12-23T16:20:49Z | - |
dc.date.available | 2021-12-23T16:20:49Z | - |
dc.date.issued | 2019 | |
dc.identifier.issn | 09379347 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/13607 | - |
dc.description.abstract | Localization of substrates in membrane proteins is an important but challenging task. In this paper, we show that deuterium electron spin echo envelopemodulation spectroscopy ((HESEEM)-H-2) combined with site-directed spin labeling is a powerful tool to localize the substrate, histidine-d5, in the ABC transporter HisQMP(2). Based on a homology model and spin label rotamer analyses, we calculated (HESEEM)-H-2 spectra for eight possible labeling positions close to the putative substrate-binding site. Experimental (HESEEM)-H-2 spectra were determined with spin labels bound either at position 169 of HisM, for which a detectable (HESEEM)-H-2 signal was calculated, or with a spin label bound at position 54 of HisQ as a negative control. The agreement between the calculated and experimental ESEEM spectra provides strong evidence for the histidine located in a binding site primarily liganded by residues of HisM as proposed by the homology model. | |
dc.description.sponsorship | Alexander von Humboldt FoundationAlexander von Humboldt Foundation; DFGGerman Research Foundation (DFG)European Commission [STE640/10, SCHN274/16-1]; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [17-54-49004]; We thank Heidi Landmesser (Humboldt Universitat zu Berlin) for excellent technical assistance. This work was supported by Alexander von Humboldt Foundation and DFG (STE640/10 to H.J.S. and SCHN274/16-1 to E.S.). Computational modeling was funded by the Russian Foundation for Basic Research grant 17-54-49004. | |
dc.language.iso | en | |
dc.publisher | SPRINGER WIEN | |
dc.relation.ispartof | APPLIED MAGNETIC RESONANCE | |
dc.subject | BINDING | |
dc.subject | DIVERSITY | |
dc.subject | ELECTRON-SPIN-ECHO | |
dc.subject | ENVELOPE MODULATION | |
dc.subject | EPR SPECTROSCOPY | |
dc.subject | INVESTIGATE | |
dc.subject | MEMBRANE | |
dc.subject | Physics | |
dc.subject | Physics, Atomic, Molecular & Chemical | |
dc.subject | PROTEINS | |
dc.subject | RADICALS | |
dc.subject | Spectroscopy | |
dc.subject | WATER CONCENTRATION | |
dc.title | ESEEM Reveals Bound Substrate Histidine in the ABC Transporter HisQMP(2) | |
dc.type | journal article | |
dc.identifier.doi | 10.1007/s00723-019-01114-y | |
dc.identifier.isi | ISI:000472013400003 | |
dc.description.volume | 50 | |
dc.description.issue | 7 | |
dc.description.startpage | 883 | |
dc.description.endpage | 893 | |
dc.contributor.orcid | 0000-0002-5888-0157 | |
dc.contributor.researcherid | AAO-1621-2020 | |
dc.contributor.researcherid | H-3791-2014 | |
dc.identifier.eissn | 16137507 | |
dc.publisher.place | SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA | |
dcterms.isPartOf.abbreviation | Appl. Magn. Reson. | |
crisitem.author.dept | Universität Osnabrück | - |
crisitem.author.dept | Universität Osnabrück | - |
crisitem.author.dept | Universität Osnabrück | - |
crisitem.author.dept | FB 04 - Physik | - |
crisitem.author.deptid | fb04 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | IsNi001 | - |
crisitem.author.netid | HeJo001 | - |
crisitem.author.netid | IvNi001 | - |
crisitem.author.netid | ScEr001 | - |
crisitem.author.netid | StHe633 | - |
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geprüft am 06.06.2024