When fathers are instant losers: homogenization of rDNA loci in recently formed Cardamine X schulzii trigenomic allopolyploid

DC FieldValueLanguage
dc.contributor.authorZozomova-Lihova, Judita
dc.contributor.authorMandakova, Terezie
dc.contributor.authorKovarikova, Alena
dc.contributor.authorMuehlhausen, Andreas
dc.contributor.authorMummenhoff, Klaus
dc.contributor.authorLysak, Martin A.
dc.contributor.authorKovarik, Ales
dc.date.accessioned2021-12-23T16:20:54Z-
dc.date.available2021-12-23T16:20:54Z-
dc.date.issued2014
dc.identifier.issn0028646X
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/13643-
dc.description.abstractRecently formed allopolyploids represent an excellent system to study the impacts of hybridization and genomic duplication on genome structure and evolution. Here we explored the 35S rRNA genes (rDNA) in the Cardamine x schulzii allohexaploid that was formed by two subsequent hybridization events within the past c. 150 yr. The rDNA loci were analyzed by cloning, next generation sequencing (NGS), RT-PCR and FISH methods. The primary C. x insueta triploid hybrid derived from C. rivularis (female) and C. amara (male) had gene ratios highly skewed towards maternal sequences. Similarly, C. x schulzii, originating from the secondary hybridization event involving C. x insueta (female) and C. pratensis (male), showed a reduction in paternal rDNA homeologs despite an excess of chromosomes inherited from C. pratensis. We also identified novel rDNA loci in C. x schulzii, suggesting that lost loci might be slowly reinstalled by translocation (but not recombination) of genes from partner genomes. Prevalent clonal propagation of allopolyploids, C. x insueta and C. x schulzii, indicates that concerted evolution of rDNA may occur in the absence of extensive meiotic cycles. Adoption of NGS in rDNA variant analysis is highly informative for deciphering the evolutionary histories of allopolyploid species with ongoing homogenization processes.
dc.description.sponsorshipEuropean Social FundEuropean Social Fund (ESF) [CZ.1.07/2.3.00/30.0037, CZ.1.07/2.3.00/20.0189]; Czech Science FoundationGrant Agency of the Czech Republic [P501/12/G090]; Slovak Research and Development AgencySlovak Research and Development Agency [APVV-0139-12]; This work was supported by the European Social Fund (project CZ.1.07/2.3.00/30.0037 and CZ.1.07/2.3.00/20.0189) and Czech Science Foundation (excellence cluster P501/12/G090), and by the Slovak Research and Development Agency (project no. APVV-0139-12 to J.Z-L.). The authors are grateful to Ulrike Coja for technical assistance with cloning analysis, the curator of the ZT (Zurich) herbarium for allowing us to study and analyze herbarium specimens from Urnerboden, Karol Marhold for assistance in the field sampling and sharing biological knowledge, and the editor and reviewers for their constructive comments. We thank Lucille Schmieding and John D. Brooker for text editing.
dc.language.isoen
dc.publisherWILEY
dc.relation.ispartofNEW PHYTOLOGIST
dc.subjectBRASSICACEAE
dc.subjectCONCERTED EVOLUTION
dc.subjectGENOME
dc.subjectHYBRIDIZATION
dc.subjecthybrids
dc.subjectPARENTAL RDNA
dc.subjectPlant Sciences
dc.subjectpolyploidy
dc.subjectrDNA
dc.subjectRIBOSOMAL DNA
dc.subjectRNA GENE SITES
dc.subjectSENECIO-CAMBRENSIS
dc.subjectSEQUENCES
dc.subjectTRAGOPOGON ASTERACEAE
dc.titleWhen fathers are instant losers: homogenization of rDNA loci in recently formed Cardamine X schulzii trigenomic allopolyploid
dc.typejournal article
dc.identifier.doi10.1111/nph.12873
dc.identifier.isiISI:000340286000010
dc.description.volume203
dc.description.issue4
dc.description.startpage1096
dc.description.endpage1108
dc.contributor.orcid0000-0002-8950-6643
dc.contributor.orcid0000-0002-8950-6643
dc.contributor.orcid0000-0003-0318-4194
dc.contributor.orcid0000-0003-2896-0698
dc.contributor.orcid0000-0001-6485-0563
dc.contributor.orcid0000-0002-8449-1593
dc.contributor.researcheridP-3151-2019
dc.contributor.researcheridH-1952-2014
dc.contributor.researcheridB-5011-2011
dc.contributor.researcheridD-7177-2012
dc.contributor.researcheridE-1365-2012
dc.identifier.eissn14698137
dc.publisher.place111 RIVER ST, HOBOKEN 07030-5774, NJ USA
dcterms.isPartOf.abbreviationNew Phytol.
dcterms.oaStatusBronze
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0002-8449-1593-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidMuKl919-
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