Vps39 Interacts with Tom40 to Establish One of Two Functionally Distinct Vacuole-Mitochondria Contact Sites
DC Element | Wert | Sprache |
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dc.contributor.author | Montoro, Ayelen Gonzalez | |
dc.contributor.author | Auffarth, Kathrin | |
dc.contributor.author | Hoenscher, Carina | |
dc.contributor.author | Bohnert, Maria | |
dc.contributor.author | Becker, Thomas | |
dc.contributor.author | Warscheid, Bettina | |
dc.contributor.author | Reggiori, Fulvio | |
dc.contributor.author | van der Laan, Martin | |
dc.contributor.author | Froehlich, Florian | |
dc.contributor.author | Ungermann, Christian | |
dc.date.accessioned | 2021-12-23T16:21:05Z | - |
dc.date.available | 2021-12-23T16:21:05Z | - |
dc.date.issued | 2018 | |
dc.identifier.issn | 15345807 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/13722 | - |
dc.description.abstract | The extensive subcellular network of membrane contact sites plays central roles in organelle biogenesis and communication, yet the precise contributions of the involved machineries remain largely enigmatic. The yeast vacuole forms a membrane contact site with mitochondria, called vacuolar and mitochondrial patch (vCLAMP). Formation of vCLAMPs involves the vacuolar Rab GTPase Ypt7 and the Ypt7-interacting Vps39 subunit of the HOPS tethering complex. Here, we uncover the general preprotein translocase of the outer membrane (TOM) subunit Tom40 as the direct binding partner of Vps39 on mitochondria. We identify Vps39 mutants defective in TOM binding, but functional for HOPS. Cells that cannot form vCLAMPs show reduced growth under stress conditions and impaired survival upon starvation. Unexpectedly, our mutant analysis revealed the existence of two functionally independent vacuole-mitochondria MCSs: one formed by the Ypt7-Vps39-Tom40 tether and a second one by Vps13-Mcp1, which is redundant with ER-mitochondrial contacts formed by ERMES. | |
dc.description.sponsorship | DFGGerman Research Foundation (DFG)European Commission [UN111/10-1, FR 3647/2-1, SFB 894, IRTG 1830]; European Molecular Biology OrganizationEuropean Molecular Biology Organization (EMBO) [EMBO ALTF 873-2015]; European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant [705853]; Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [BE 4679/2-1, SFB 746]; Deutsche Forschungsgemeinschaft (Research Training Group RTG 2202)German Research Foundation (DFG); Excellence Initiative of the German Federal Government [EXC 294 BIOSS]; Excellence Initiative of the German State Government [EXC 294 BIOSS]; [Sonderforschungsbereich (SFB) 944]; [SFB 944]; We thank Maya Schuldiner, Benoit Kornmann, and Bernard Guiard for strains and plasmids; Max-Hinderk Schuler for the basic characterization of yeast mutants; and all members of the Ungermann group for helpful discussions. We thank Stefan Walter for support with all mass spectrometry-based experiments. This work was funded by a grant of the DFG to C.U. (UN111/10-1) with additional support by the Sonderforschungsbereich (SFB) 944. A.G.M. is recipient of a postdoctoral fellowship of the European Molecular Biology Organization (EMBO ALTF 873-2015) and a young investigator grant of the SFB 944. F.F. is supported by a grant from the DFG (FR 3647/2-1). M.B. is supported by the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 705853. Research from T.B. is supported by grants from the Deutsche Forschungsgemeinschaft (BE 4679/2-1; SFB 746, Research Training Group RTG 2202). B.W. and T.B. are supported by the Excellence Initiative of the German Federal & State Governments (EXC 294 BIOSS). M.v.d.L. is supported by grants of the DFG (SFB 894 and the IRTG 1830). | |
dc.language.iso | en | |
dc.publisher | CELL PRESS | |
dc.relation.ispartof | DEVELOPMENTAL CELL | |
dc.subject | Cell Biology | |
dc.subject | CELLULAR-METABOLISM | |
dc.subject | Developmental Biology | |
dc.subject | ER-MITOCHONDRIA | |
dc.subject | HOPS TETHERING COMPLEX | |
dc.subject | MOLECULAR ARCHITECTURE | |
dc.subject | OUTER-MEMBRANE | |
dc.subject | PREPROTEIN TRANSLOCASE | |
dc.subject | PROTEIN | |
dc.subject | RAB INTERACTIONS | |
dc.subject | SACCHAROMYCES-CEREVISIAE | |
dc.subject | YEAST | |
dc.title | Vps39 Interacts with Tom40 to Establish One of Two Functionally Distinct Vacuole-Mitochondria Contact Sites | |
dc.type | journal article | |
dc.identifier.doi | 10.1016/j.devcel.2018.05.011 | |
dc.identifier.isi | ISI:000435092700011 | |
dc.description.volume | 45 | |
dc.description.issue | 5 | |
dc.description.startpage | 621+ | |
dc.contributor.orcid | 0000-0002-8154-555X | |
dc.contributor.orcid | 0000-0003-2652-2686 | |
dc.contributor.orcid | 0000-0003-0789-9912 | |
dc.contributor.orcid | 0000-0001-5096-1975 | |
dc.contributor.orcid | 0000-0001-8307-2189 | |
dc.contributor.researcherid | AAS-9404-2020 | |
dc.contributor.researcherid | U-8327-2019 | |
dc.contributor.researcherid | AAB-7704-2019 | |
dc.identifier.eissn | 18781551 | |
dc.publisher.place | 50 HAMPSHIRE ST, FLOOR 5, CAMBRIDGE, MA 02139 USA | |
dcterms.isPartOf.abbreviation | Dev. Cell | |
dcterms.oaStatus | Bronze | |
crisitem.author.dept | Sonderforschungsbereich 944: Physiologie und Dynamik zellulärer Mikrokompartimente | - |
crisitem.author.deptid | organisation19 | - |
crisitem.author.orcid | 0000-0001-8307-2189 | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | FrFl166 | - |
crisitem.author.netid | UnCh999 | - |
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