The I-BAR protein Ivy1 is an effector of the Rab7 GTPase Ypt7 involved in vacuole membrane homeostasis
DC Element | Wert | Sprache |
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dc.contributor.author | Numrich, Johannes | |
dc.contributor.author | Peli-Gulli, Marie-Pierre | |
dc.contributor.author | Arlt, Henning | |
dc.contributor.author | Sardu, Alessandro | |
dc.contributor.author | Griffith, Janice | |
dc.contributor.author | Levine, Tim | |
dc.contributor.author | Engelbrecht-Vandre, Siegfried | |
dc.contributor.author | Reggiori, Fulvio | |
dc.contributor.author | De Virgilio, Claudio | |
dc.contributor.author | Ungermann, Christian | |
dc.date.accessioned | 2021-12-23T16:21:06Z | - |
dc.date.available | 2021-12-23T16:21:06Z | - |
dc.date.issued | 2015 | |
dc.identifier.issn | 00219533 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/13724 | - |
dc.description.abstract | Membrane fusion at the vacuole depends on a conserved machinery that includes SNAREs, the Rab7 homolog Ypt7 and its effector HOPS. Here, we demonstrate that Ypt7 has an unexpected additional function by controlling membrane homeostasis and nutrient-dependent signaling on the vacuole surface. We show that Ivy1, the yeast homolog of mammalian missing-in-metastasis (MIM), is a vacuolar effector of Ypt7-GTP and interacts with the EGO/ragulator complex, an activator of the target of rapamycin kinase complex 1 (TORC1) on vacuoles. Loss of Ivy1 does not affect EGO vacuolar localization and function. In combination with the deletion of individual subunits of the V-ATPase, however, we observed reduced TORC1 activity and massive enlargement of the vacuole surface. Consistent with this, Ivy1 localizes to invaginations at the vacuole surface and on liposomes in a phosphoinositide- and Ypt7-GTP-controlled manner, which suggests a role in microautophagy. Our data, thus, reveal that Ivy1 is a novel regulator of vacuole membrane homeostasis with connections to TORC1 signaling. | |
dc.description.sponsorship | DFGGerman Research Foundation (DFG)European Commission [UN111/7-1, SFB 944]; Hans-Muhlenhoff foundation; Swiss National FoundationSwiss National Science Foundation (SNSF); ECHO [700.59.003]; ALW Open Program [821.02.017, 822.02.014]; DFG-NWO cooperation [DN82-303]; ZonMW VICI [016.130.606]; BBSRCUK Research & Innovation (UKRI)Biotechnology and Biological Sciences Research Council (BBSRC) [BB/M011801/1] Funding Source: UKRI; We thank Markus Babst for suggestions and members of the Ungermann lab for discussions. This work was supported by the DFG (UN111/7-1), the SFB 944 (project P11) and by the Hans-Muhlenhoff foundation (to C.U.), and the Swiss National Foundation (to C.D.V.). F.R. is supported by ECHO (700.59.003), ALW Open Program (821.02.017 and 822.02.014), DFG-NWO cooperation (DN82-303) and ZonMW VICI (016.130.606) grants. | |
dc.language.iso | en | |
dc.publisher | COMPANY OF BIOLOGISTS LTD | |
dc.relation.ispartof | JOURNAL OF CELL SCIENCE | |
dc.subject | AUTOPHAGOSOME FORMATION | |
dc.subject | BINDING PROTEIN | |
dc.subject | Cell Biology | |
dc.subject | CONTROLS TORC1 | |
dc.subject | EGO COMPLEX | |
dc.subject | HOMOTYPIC FUSION | |
dc.subject | HOPS TETHERING COMPLEX | |
dc.subject | Ivy1 | |
dc.subject | LYSOSOMAL SURFACE | |
dc.subject | NUCLEOTIDE EXCHANGE | |
dc.subject | SNARE CHAPERONES | |
dc.subject | TORC1 | |
dc.subject | Vacuole biogenesis | |
dc.subject | Vps33 | |
dc.subject | YEAST SACCHAROMYCES-CEREVISIAE | |
dc.subject | Ypt7 | |
dc.title | The I-BAR protein Ivy1 is an effector of the Rab7 GTPase Ypt7 involved in vacuole membrane homeostasis | |
dc.type | journal article | |
dc.identifier.doi | 10.1242/jcs.164905 | |
dc.identifier.isi | ISI:000358944500007 | |
dc.description.volume | 128 | |
dc.description.issue | 13 | |
dc.description.startpage | 2278 | |
dc.description.endpage | 2292 | |
dc.contributor.orcid | 0000-0001-8826-4323 | |
dc.contributor.orcid | 0000-0002-7231-0775 | |
dc.contributor.orcid | 0000-0003-2652-2686 | |
dc.contributor.orcid | 0000-0001-6395-9620 | |
dc.contributor.researcherid | U-8327-2019 | |
dc.contributor.researcherid | B-4707-2012 | |
dc.identifier.eissn | 14779137 | |
dc.publisher.place | BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE CB4 4DL, CAMBS, ENGLAND | |
dcterms.isPartOf.abbreviation | J. Cell Sci. | |
dcterms.oaStatus | Green Submitted, Green Published, Bronze | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0001-6395-9620 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | ArHe830 | - |
crisitem.author.netid | UnCh999 | - |
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