Optical manipulation of sphingolipid biosynthesis using photoswitchable ceramides

DC FieldValueLanguage
dc.contributor.authorKol, Matthijs
dc.contributor.authorWilliams, Ben
dc.contributor.authorToombs-Ruane, Henry
dc.contributor.authorFranquelim, Henri G.
dc.contributor.authorKorneev, Sergei
dc.contributor.authorSchroeer, Christian
dc.contributor.authorSchwille, Petra
dc.contributor.authorTrauner, Dirk
dc.contributor.authorHolthuis, Joost C. M.
dc.contributor.authorFrank, James A.
dc.date.accessioned2021-12-23T16:21:07Z-
dc.date.available2021-12-23T16:21:07Z-
dc.date.issued2019
dc.identifier.issn2050084X
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/13735-
dc.description.abstractCeramides are central intermediates of sphingolipid metabolism that also function as potent messengers in stress signaling and apoptosis. Progress in understanding how ceramides execute their biological roles is hampered by a lack of methods to manipulate their cellular levels and metabolic fate with appropriate spatiotemporal precision. Here, we report on clickable, azobenzene-containing ceramides, caCers, as photoswitchable metabolic substrates to exert optical control over sphingolipid production in cells. Combining atomic force microscopy on model bilayers with metabolic tracing studies in cells, we demonstrate that light-induced alterations in the lateral packing of caCers lead to marked differences in their metabolic conversion by sphingomyelin synthase and glucosylceramide synthase. These changes in metabolic rates are instant and reversible over several cycles of photoswitching. Our findings disclose new opportunities to probe the causal roles of ceramides and their metabolic derivatives in a wide array of sphingolipid-dependent cellular processes with the spatiotemporal precision of light.
dc.description.sponsorshipDeutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [SFB1032, SFB944]; Natural Sciences and Engineering Research Council of CanadaNatural Sciences and Engineering Research Council of Canada (NSERC)CGIAR; university of Osnabruck; Deutsche Forschungsgemeinschaft SFB1032 Henri G Franquelim Petra Schwille Dirk Trauner James A Frank; Natural Sciences and Engineering Research Council of Canada Ben Williams Deutsche Forschungsgemeinschaft SFB944 Matthijs Kol Joost CM Holthuis; university of Osnabruck Incentive Award Matthijs Kol; The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
dc.language.isoen
dc.publisherELIFE SCIENCES PUBLICATIONS LTD
dc.relation.ispartofELIFE
dc.subjectASYMMETRIC-SYNTHESIS
dc.subjectBiology
dc.subjectCROSS-METATHESIS
dc.subjectEFFICIENT
dc.subjectGROWTH
dc.subjectINHIBITORS
dc.subjectLife Sciences & Biomedicine - Other Topics
dc.subjectLIPIDS
dc.subjectMETABOLISM
dc.subjectSPHINGOMYELIN SYNTHASES
dc.subjectSPHINGOSINE
dc.subjectTRANSPORT
dc.titleOptical manipulation of sphingolipid biosynthesis using photoswitchable ceramides
dc.typejournal article
dc.identifier.doi10.7554/eLife.43230
dc.identifier.isiISI:000459461000001
dc.description.volume8
dc.contributor.orcid0000-0003-3068-6501
dc.contributor.orcid0000-0002-6106-4847
dc.contributor.orcid0000-0001-6229-4276
dc.contributor.orcid0000-0003-1483-4981
dc.contributor.orcid0000-0001-8912-1586
dc.contributor.researcheridA-4983-2010
dc.contributor.researcheridAAL-9157-2020
dc.contributor.researcheridP-4985-2017
dc.publisher.placeSHERATON HOUSE, CASTLE PARK, CAMBRIDGE, CB3 0AX, ENGLAND
dcterms.isPartOf.abbreviationeLife
dcterms.oaStatusGreen Published, gold, Green Submitted
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidKoSe681-
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