Three-Dimensional Interfacing of Cells with Hierarchical Silicon Nano/Microstructures for Midinfrared Interrogation of In Situ Captured Proteins
DC Element | Wert | Sprache |
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dc.contributor.author | Flesch, Julia | |
dc.contributor.author | Bettenhausen, Maximilian | |
dc.contributor.author | Kazmierczak, Marcin | |
dc.contributor.author | Klesse, Wolfgang M. | |
dc.contributor.author | Skibitzki, Oliver | |
dc.contributor.author | Psathaki, Olympia E. | |
dc.contributor.author | Kurre, Rainer | |
dc.contributor.author | Capellini, Giovanni | |
dc.contributor.author | Guha, Subhajit | |
dc.contributor.author | Schroeder, Thomas | |
dc.contributor.author | Witzigmann, Bernd | |
dc.contributor.author | You, Changjiang | |
dc.contributor.author | Piehler, Jacob | |
dc.date.accessioned | 2021-12-23T16:21:11Z | - |
dc.date.available | 2021-12-23T16:21:11Z | - |
dc.date.issued | 2021 | |
dc.identifier.issn | 19448244 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/13764 | - |
dc.description.abstract | Label-free optical detection of biomolecules is currently limited by a lack of specificity rather than sensitivity. To exploit the much more characteristic refractive index dispersion in the mid-infrared (IR) regime, we have engineered three-dimensional IR-resonant silicon micropillar arrays (Si-MPAs) for protein sensing. By exploiting the unique hierarchical nano- and microstructured design of these Si-MPAs attained by CMOS-compatible silicon-based microfabrication processes, we achieved an optimized interrogation of surface protein binding. Based on spatially resolved surface functionalization, we demonstrate controlled three-dimensional interfacing of mammalian cells with Si-MPAs. Spatially controlled surface functionalization for site-specific protein immobilization enabled efficient targeting of soluble and membrane proteins into sensing hotspots directly from cells cultured on Si-MPAs. Protein binding to Si-MPA hotspots at submonolayer level was unambiguously detected by conventional Fourier transform IR spectroscopy. The compatibility with cost-effective CMOS-based microfabrication techniques readily allows integration of this novel IR transducer into fully fledged bioanalytical microdevices for selective and sensitive protein sensing. | |
dc.description.sponsorship | DFGGerman Research Foundation (DFG)European Commission [SPP 1857, PI 405/9, PI405/10, SCHR 1123/11, WI 3317/6, CA 1474/4, PI 405/14-1]; Osnabruck University; We thank G. Hikade and H. Kenneweg for technical support. This project was supported by funding from the DFG to B.W., T.S., G.C., and J.P. (SPP 1857 ESSENCE, PI 405/9, PI405/10, SCHR 1123/11, WI 3317/6, CA 1474/4) and by intramural funding from Osnabruck University (profile line ``Integrated Science''). The DFG-funded Integrated Bioimaging Facility (PI 405/14-1) provided access to advanced fluorescence and electron microscopy techniques. | |
dc.language.iso | en | |
dc.publisher | AMER CHEMICAL SOC | |
dc.relation.ispartof | ACS APPLIED MATERIALS & INTERFACES | |
dc.subject | cell-sensor-interfacing | |
dc.subject | CMOS microfabrication | |
dc.subject | in situ protein immobilization | |
dc.subject | IR refractive index sensing | |
dc.subject | label-free protein sensing | |
dc.subject | Materials Science | |
dc.subject | Materials Science, Multidisciplinary | |
dc.subject | Nanoscience & Nanotechnology | |
dc.subject | Science & Technology - Other Topics | |
dc.subject | SPECTROSCOPY | |
dc.title | Three-Dimensional Interfacing of Cells with Hierarchical Silicon Nano/Microstructures for Midinfrared Interrogation of In Situ Captured Proteins | |
dc.type | journal article | |
dc.identifier.doi | 10.1021/acsami.0c22421 | |
dc.identifier.isi | ISI:000623228500020 | |
dc.description.volume | 13 | |
dc.description.issue | 7 | |
dc.description.startpage | 8049 | |
dc.description.endpage | 8059 | |
dc.contributor.orcid | 0000-0002-7839-6397 | |
dc.identifier.eissn | 19448252 | |
dc.publisher.place | 1155 16TH ST, NW, WASHINGTON, DC 20036 USA | |
dcterms.isPartOf.abbreviation | ACS Appl. Mater. Interfaces | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.dept | Sonderforschungsbereich 944: Physiologie und Dynamik zellulärer Mikrokompartimente | - |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.deptid | organisation19 | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-6872-6567 | - |
crisitem.author.orcid | 0000-0002-7839-6397 | - |
crisitem.author.orcid | 0000-0002-2143-2270 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | KuRa617 | - |
crisitem.author.netid | YoCh745 | - |
crisitem.author.netid | PiJa938 | - |
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geprüft am 12.05.2024