Multiple strategies to prevent oxidative stress in Arabidopsis plants lacking the malate valve enzyme NADP-malate dehydrogenase

DC FieldValueLanguage
dc.contributor.authorHebbelmann, Inga
dc.contributor.authorSelinski, Jennifer
dc.contributor.authorWehmeyer, Corinna
dc.contributor.authorGoss, Tatjana
dc.contributor.authorVoss, Ingo
dc.contributor.authorMulo, Paula
dc.contributor.authorKangasjarvi, Saijaliisa
dc.contributor.authorAro, Eva-Mari
dc.contributor.authorOelze, Marie-Luise
dc.contributor.authorDietz, Karl-Josef
dc.contributor.authorNunes-Nesi, Adriano
dc.contributor.authorDo, Phuc T.
dc.contributor.authorFernie, Alisdair R.
dc.contributor.authorTalla, Sai K.
dc.contributor.authorRaghavendra, Agepati S.
dc.contributor.authorLinke, Vera
dc.contributor.authorScheibe, Renate
dc.date.accessioned2021-12-23T16:21:26Z-
dc.date.available2021-12-23T16:21:26Z-
dc.date.issued2012
dc.identifier.issn00220957
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/13872-
dc.description.abstractThe nuclear-encoded chloroplast NADP-dependent malate dehydrogenase (NADP-MDH) is a key enzyme controlling the malate valve, to allow the indirect export of reducing equivalents. Arabidopsis thaliana (L.) Heynh. T-DNA insertion mutants of NADP-MDH were used to assess the role of the light-activated NADP-MDH in a typical C-3 plant. Surprisingly, even when exposed to high-light conditions in short days, nadp-mdh knockout mutants were phenotypically indistinguishable from the wild type. The photosynthetic performance and typical antioxidative systems, such as the Beck-Halliwell-Asada pathway, were barely affected in the mutants in response to high-light treatment. The reactive oxygen species levels remained low, indicating the apparent absence of oxidative stress, in the mutants. Further analysis revealed a novel combination of compensatory mechanisms in order to maintain redox homeostasis in the nadp-mdh plants under high-light conditions, particularly an increase in the NTRC/2-Cys peroxiredoxin (Prx) system in chloroplasts. There were indications of adjustments in extra-chloroplastic components of photorespiration and proline levels, which all could dissipate excess reducing equivalents, sustain photosynthesis, and prevent photoinhibition in nadp-mdh knockout plants. Such metabolic flexibility suggests that the malate valve acts in concert with other NADPH-consuming reactions to maintain a balanced redox state during photosynthesis under high-light stress in wild-type plants.
dc.description.sponsorshipDeutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [EM166/1]; Department of Science and Technology-Deutscher Akademischer Austauschdienst (DAAD); DAAD-Finland; DFGGerman Research Foundation (DFG)European Commission; Academy of FinlandAcademy of FinlandEuropean Commission [118637, 8133293, 130075, 130595]; This study was financially supported by the Deutsche Forschungsgemeinschaft to V.L. and R.S. (EM166/1), grants from the Department of Science and Technology-Deutscher Akademischer Austauschdienst (DAAD, to A.S.R. and R.S.), the DAAD-Finland program (to R.S. and E.-M.A.), and by a DFG-Mercator Visiting Professorship to A.S.R. Funds were also received from the Academy of Finland to E.-M.A. (118637, 8133293), P.M. (130075), and S.K. (130595). The authors thank Dr Francisco Javier Cejudo (Sevilla) for the gift of anti-NTRC serum as well as probes for NTRC, and Dr Eevi Rintamaki for the antibody against Arabidopsis APXs. We thank Professor Hermann Bauwe (Rostock) for probes and antiserum against GDC (P-protein). We also thank Guiseppe Forlani (Ferrara, Italy) for providing P5C, and Dietmar Funck (Konstanz, Germany) for advice concerning proline metabolic enzyme measurements. Thanks are due to Silke Walter for expert technical assistance. We also thank Heike Wolf-Wibbelmann and Kirsten Jager for cultivating the plants, and Heike Schwiderski and Nicolas Konig for their help with the preparation of the manuscript.
dc.language.isoen
dc.publisherOXFORD UNIV PRESS
dc.relation.ispartofJOURNAL OF EXPERIMENTAL BOTANY
dc.subjectANTIOXIDANT DEFENSES
dc.subjectASCORBATE
dc.subjectCHLOROPLAST
dc.subjectGLUTATHIONE
dc.subjectLIGHT
dc.subjectMalate valve
dc.subjectMESOPHYLL PROTOPLASTS
dc.subjectNADP-malate dehydrogenase
dc.subjectoxidative stress
dc.subjectPHOTORESPIRATION
dc.subjectPlant Sciences
dc.subjectpoising mechanisms
dc.subjectREDOX HOMEOSTASIS
dc.subjectTHALIANA
dc.subjectTHIOREDOXIN REDUCTASE
dc.titleMultiple strategies to prevent oxidative stress in Arabidopsis plants lacking the malate valve enzyme NADP-malate dehydrogenase
dc.typejournal article
dc.identifier.doi10.1093/jxb/err386
dc.identifier.isiISI:000300238400032
dc.description.volume63
dc.description.issue3
dc.description.startpage1445
dc.description.endpage1459
dc.contributor.orcid0000-0002-7376-4947
dc.contributor.orcid0000-0002-2922-1435
dc.contributor.orcid0000-0002-7605-8546
dc.contributor.orcid0000-0003-0311-2182
dc.contributor.orcid0000-0002-2893-8223
dc.contributor.orcid0000-0002-1247-7282
dc.contributor.orcid0000-0002-8728-3204
dc.contributor.orcid0000-0002-9581-9355
dc.contributor.researcheridC-2166-2011
dc.contributor.researcheridQ-8664-2017
dc.contributor.researcheridH-9013-2013
dc.contributor.researcheridD-6430-2013
dc.contributor.researcheridB-6029-2009
dc.contributor.researcheridA-2318-2014
dc.identifier.eissn14602431
dc.publisher.placeGREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
dcterms.isPartOf.abbreviationJ. Exp. Bot.
dcterms.oaStatusGreen Published, hybrid, Green Submitted
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0002-6140-6181-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidScRe288-
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