8-N-3-3 `-biotnyl-ATP, a novel monofunctional reagent: Differences in the F-1- and V-1-ATPases by means of the ATP analogue

DC FieldValueLanguage
dc.contributor.authorSchafer, HJ
dc.contributor.authorCoskun, U
dc.contributor.authorEger, O
dc.contributor.authorGodovac-Zimmermann, J
dc.contributor.authorWieczorek, H
dc.contributor.authorKagawa, Y
dc.contributor.authorGruber, G
dc.date.accessioned2021-12-23T16:21:53Z-
dc.date.available2021-12-23T16:21:53Z-
dc.date.issued2001
dc.identifier.issn0006291X
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/14064-
dc.description.abstractA novel photoaffinity label, 8-N-3-3'-biotinyl-ATP, has been synthesized. The introduction of an additional biotin residue is advantageous for easy detection of labeled proteins. This could be first tested by reaction with the F-1-ATPase from the thermophilic bacterium PS3 (TF1). UV irradiation of TF1 in the presence of 8-N-3-3'-biotinyl-ATP results in a nucleotide-dependent binding of the analogue in the noncatalytic a and the catalytic beta subunits of TF1, demonstrating the suitability of this analogue as a potential photoaffinity label. Reaction with the V-1-ATPase, however, led to labeling of subunit E, which has been suggested as a structural and functional homologue of the gamma subunit of the F-ATPases. MALDI-TOF mass spectrometry has been used to map the regions of subunit E involved in the binding of 8-N-3-3'-biotiinyl-ATP. (C) 2001 Academic Press.
dc.language.isoen
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE
dc.relation.ispartofBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
dc.subject5'-TRIPHOSPHATE
dc.subject8-AZIDO-ATP
dc.subjectBiochemistry & Molecular Biology
dc.subjectBiophysics
dc.subjectCOATED VESICLE
dc.subjectF-1-ATPase
dc.subjectGAMMA-SUBUNIT
dc.subjectH+-translocating vacuolar-type ATPase
dc.subjectHEART MITOCHONDRIAL ATPASE
dc.subjectManduca sexta
dc.subjectNUCLEOTIDE-BINDING-SITES
dc.subjectPHOTOAFFINITY LABEL
dc.subjectphotoaffinity labeling
dc.subjectRESOLUTION
dc.subjectthermophilic bacterium PS3
dc.subjectV-1-ATPase
dc.subjectV-ATPASE
dc.subjectVACUOLAR (H+)-ATPASE
dc.title8-N-3-3 `-biotnyl-ATP, a novel monofunctional reagent: Differences in the F-1- and V-1-ATPases by means of the ATP analogue
dc.typejournal article
dc.identifier.doi10.1006/bbrc.2001.5502
dc.identifier.isiISI:000170966200057
dc.description.volume286
dc.description.issue5
dc.description.startpage1218
dc.description.endpage1227
dc.contributor.orcid0000-0003-4375-3144
dc.contributor.researcheridF-4683-2011
dc.contributor.researcheridE-6863-2010
dc.identifier.eissn10902104
dc.publisher.place525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
dcterms.isPartOf.abbreviationBiochem. Biophys. Res. Commun.
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidWiHe990-
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