Ligand-induced conformational capture of a synthetic tetracycline riboswitch revealed by pulse EPR
DC Element | Wert | Sprache |
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dc.contributor.author | Wunnicke, Dorith | |
dc.contributor.author | Strohbach, Denise | |
dc.contributor.author | Weigand, Julia E. | |
dc.contributor.author | Appel, Bettina | |
dc.contributor.author | Feresin, Emiliano | |
dc.contributor.author | Suess, Beatrix | |
dc.contributor.author | Mueller, Sabine | |
dc.contributor.author | Steinhoff, Heinz-Juergen | |
dc.date.accessioned | 2021-12-23T16:21:58Z | - |
dc.date.available | 2021-12-23T16:21:58Z | - |
dc.date.issued | 2011 | |
dc.identifier.issn | 13558382 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/14098 | - |
dc.description.abstract | RNA aptamers are in vitro-selected binding domains that recognize their respective ligand with high affinity and specificity. They are characterized by complex three-dimensional conformations providing preformed binding pockets that undergo conformational changes upon ligand binding. Small molecule-binding aptamers have been exploited as synthetic riboswitches for conditional gene expression in various organisms. In the present study, double electron-electron resonance (DEER) spectroscopy combined with site-directed spin labeling was used to elucidate the conformational transition of a tetracycline aptamer upon ligand binding. Different sites were selected for post-synthetic introduction of either the (1-oxyl-2,2,5,5-tetramethylpyrroline- 3-methyl) methanethiosulfonate by reaction with a 4-thiouridine modified RNA or of 4-isocyanato-2,6-tetramethylpiperidyl- N-oxid spin label by reaction with 2'-aminouridine modified RNA. The results of the DEER experiments indicate the presence of a thermodynamic equilibrium between two aptamer conformations in the free state and capture of one conformation upon tetracycline binding. | |
dc.description.sponsorship | VolkswagenstiftungVolkswagen [I/79 950-952]; We gratefully acknowledge funding by the Volkswagenstiftung (I/79 950-952). | |
dc.language.iso | en | |
dc.publisher | COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT | |
dc.relation.ispartof | RNA | |
dc.subject | APTAMERS | |
dc.subject | BINDING RIBOSWITCH | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | DEER | |
dc.subject | DISTANCE MEASUREMENTS | |
dc.subject | DYNAMICS | |
dc.subject | ELECTRON-PARAMAGNETIC-RESONANCE | |
dc.subject | ENERGY-TRANSFER | |
dc.subject | ESR | |
dc.subject | NUCLEIC-ACIDS | |
dc.subject | RNA | |
dc.subject | RNA folding | |
dc.subject | SITE | |
dc.subject | site-directed spin labeling | |
dc.subject | SPECTROSCOPY | |
dc.subject | synthetic riboswitch | |
dc.subject | tetracycline aptamer | |
dc.title | Ligand-induced conformational capture of a synthetic tetracycline riboswitch revealed by pulse EPR | |
dc.type | journal article | |
dc.identifier.doi | 10.1261/rna.2222811 | |
dc.identifier.isi | ISI:000285338200018 | |
dc.description.volume | 17 | |
dc.description.issue | 1 | |
dc.description.startpage | 182 | |
dc.description.endpage | 188 | |
dc.contributor.orcid | 0000-0002-8385-7274 | |
dc.contributor.orcid | 0000-0002-5888-0157 | |
dc.contributor.orcid | 0000-0003-4247-1348 | |
dc.contributor.researcherid | C-1909-2013 | |
dc.contributor.researcherid | H-3791-2014 | |
dc.contributor.researcherid | D-2788-2017 | |
dc.contributor.researcherid | D-6073-2011 | |
dc.identifier.eissn | 14699001 | |
dc.publisher.place | 1 BUNGTOWN RD, COLD SPRING HARBOR, NY 11724 USA | |
dcterms.isPartOf.abbreviation | RNA | |
dcterms.oaStatus | Bronze, Green Published | |
crisitem.author.dept | FB 04 - Physik | - |
crisitem.author.deptid | fb04 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | StHe633 | - |
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geprüft am 06.06.2024