Lipid dynamics in nanoparticles formed by maleic acid-containing copolymers: EPR spectroscopy and molecular dynamics simulations
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Colbasevici, Alexandr | |
dc.contributor.author | Voskoboynikova, Natalia | |
dc.contributor.author | Orekhov, Philipp S. | |
dc.contributor.author | Bozdaganyan, Marine E. | |
dc.contributor.author | Karlova, Maria G. | |
dc.contributor.author | Sokolova, Olga S. | |
dc.contributor.author | Klare, Johann P. | |
dc.contributor.author | Mulkidjanian, Armen Y. | |
dc.contributor.author | Shaitan, V, Konstantin | |
dc.contributor.author | Steinhoff, Heinz-Juergen | |
dc.date.accessioned | 2021-12-23T16:22:00Z | - |
dc.date.available | 2021-12-23T16:22:00Z | - |
dc.date.issued | 2020 | |
dc.identifier.issn | 00052736 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/14116 | - |
dc.description.abstract | Amphiphilic maleic acid-containing copolymers account for a recent methodical breakthrough in the study of membrane proteins. Their application enables a detergent-free extraction of membrane proteins from lipid bilayers, yielding stable water-soluble, discoidal lipid bilayer particles with incorporated proteins, which are wrapped with copolymers. Although many studies confirm the potential of this approach for membrane protein research, the interactions between the maleic acid-containing copolymers and extracted lipids, as well as possible effects of the copolymers on lipid-embedded proteins deserve further scrutinization. Here, we combine electron paramagnetic resonance spectroscopy and coarse-grain molecular dynamics simulations to compare the distribution and dynamics of lipids in lipid particles of phospholipid bilayers encased either by an aliphatic diisobutylene/maleic acid copolymer (DIBMALPs) or by an aromatic styrene/maleic acid copolymer (SMALPs). Nitroxides located at the 5th, 12th or 16th carbon atom positions in phosphatidylcholine-based spin labels experience restrictions of their reorientational motion depending on the type of encasing copolymer. The dynamics of the lipids was less constrained in DIBMALPs than in SMALPs with the affinity of spin labeled lipids to the polymeric rim being more pronounced in SMALPs. | |
dc.description.sponsorship | German Research Foundation (DFG)German Research Foundation (DFG) [STE640/15]; Ostpartnerschaften programm of DAAD [18-504-12045]; RFBRRussian Foundation for Basic Research (RFBR) [18-504-12045]; Ministry of Science and Higher Education of Russia [14.619.21.0014]; Program of Leading Scientific Schools ``Depository of the Living Systems''; This work was supported by German Research Foundation (DFG, STE640/15) to H.J.S., the Ostpartnerschaften programm of DAAD and RFBR grant no. 18-504-12045 to K.V.S. Electron microscopy was performed at the special scientific facility ``3D Electron Microscopy and Spectroscopy'' (supported by Ministry of Science and Higher Education of Russia, under agreement #14.619.21.0014) of Lomonosov Moscow State University. K.V.S. and O.S.S. were supported in part by the Program of Leading Scientific Schools ``Depository of the Living Systems'' in the framework of the Lomonosov Moscow State University Development Program. The samples for TEM imaging were prepared in the electron microscopy (EM) unit of Integrated Bioimaging Facility (iBiOs) at Center of Cellular Nanoanalytics (CellNanOs), University of Osnabruck. We are very thankful to Dr. Katherina Psathaki for the support. Molecular dynamics simulations were performed using facilities of FRCCP RAS (state task AAA-A19-119012990175-9). | |
dc.language.iso | en | |
dc.publisher | ELSEVIER | |
dc.relation.ispartof | BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | |
dc.subject | BILAYER NANODISCS | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | Biophysics | |
dc.subject | COARSE-GRAINED MODEL | |
dc.subject | Coarse-grained molecular dynamics | |
dc.subject | DIBMA | |
dc.subject | FREE PURIFICATION | |
dc.subject | Liposome | |
dc.subject | MARTINI FORCE-FIELD | |
dc.subject | MEMBRANE-PROTEINS | |
dc.subject | Nitroxide spin label | |
dc.subject | ORGANIZATION | |
dc.subject | Phospholipid bilayer | |
dc.subject | RESONANCE | |
dc.subject | SMALP | |
dc.subject | SOLUBILIZATION | |
dc.subject | SPECTRA | |
dc.subject | SPIN-LABELED PHOSPHOLIPIDS | |
dc.title | Lipid dynamics in nanoparticles formed by maleic acid-containing copolymers: EPR spectroscopy and molecular dynamics simulations | |
dc.type | journal article | |
dc.identifier.doi | 10.1016/j.bbamem.2020.183207 | |
dc.identifier.isi | ISI:000528194700016 | |
dc.description.volume | 1862 | |
dc.description.issue | 5 | |
dc.contributor.orcid | 0000-0002-5888-0157 | |
dc.contributor.orcid | 0000-0002-5761-5968 | |
dc.contributor.orcid | 0000-0003-4078-4762 | |
dc.contributor.orcid | 0000-0003-4678-232X | |
dc.contributor.researcherid | H-3791-2014 | |
dc.contributor.researcherid | AAK-2153-2021 | |
dc.contributor.researcherid | ABF-2126-2020 | |
dc.contributor.researcherid | AAH-3608-2021 | |
dc.contributor.researcherid | C-1428-2009 | |
dc.contributor.researcherid | ABH-3544-2020 | |
dc.contributor.researcherid | V-5214-2017 | |
dc.contributor.researcherid | AAM-8289-2020 | |
dc.identifier.eissn | 18792642 | |
dc.publisher.place | RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS | |
dcterms.isPartOf.abbreviation | Biochim. Biophys. Acta-Biomembr. | |
dcterms.oaStatus | Bronze | |
crisitem.author.dept | FB 04 - Physik | - |
crisitem.author.dept | FB 04 - Physik | - |
crisitem.author.deptid | fb04 | - |
crisitem.author.deptid | fb04 | - |
crisitem.author.orcid | 0000-0003-2317-0144 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | VoNa568 | - |
crisitem.author.netid | StHe633 | - |
Seitenaufrufe
4
Letzte Woche
0
0
Letzter Monat
1
1
geprüft am 06.06.2024