Lipid Dynamics in Diisobutylene-Maleic Acid (DIBMA) Lipid Particles in Presence of Sensory Rhodopsin II

DC FieldValueLanguage
dc.contributor.authorVoskoboynikova, Natalia
dc.contributor.authorOrekhov, Philipp
dc.contributor.authorBozdaganyan, Marine
dc.contributor.authorKodde, Felix
dc.contributor.authorRademacher, Malte
dc.contributor.authorSchowe, Maurice
dc.contributor.authorBudke-Gieseking, Annette
dc.contributor.authorBrickwedde, Britta
dc.contributor.authorPsathaki, Olympia-Ekaterini
dc.contributor.authorMulkidjanian, Armen Y.
dc.contributor.authorCosentino, Katia
dc.contributor.authorShaitan, Konstantin V.
dc.contributor.authorSteinhoff, Heinz-Juergen
dc.date.accessioned2021-12-23T16:22:13Z-
dc.date.available2021-12-23T16:22:13Z-
dc.date.issued2021
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/14213-
dc.description.abstractAmphiphilic diisobutylene/maleic acid (DIBMA) copolymers extract lipid-encased membrane proteins from lipid bilayers in a detergent-free manner, yielding nanosized, discoidal DIBMA lipid particles (DIBMALPs). Depending on the DIBMA/lipid ratio, the size of DIBMALPs can be broadly varied which makes them suitable for the incorporation of proteins of different sizes. Here, we examine the influence of the DIBMALP sizes and the presence of protein on the dynamics of encased lipids. As shown by a set of biophysical methods, the stability of DIBMALPs remains unaffected at different DIBMA/lipid ratios. Coarse-grained molecular dynamics simulations confirm the formation of viable DIBMALPs with an overall size of up to 35 nm. Electron paramagnetic resonance spectroscopy of nitroxides located at the 5th, 12th or 16th carbon atom positions in phosphatidylcholine-based spin labels reveals that the dynamics of enclosed lipids are not altered by the DIBMALP size. The presence of the membrane protein sensory rhodopsin II from Natronomonas pharaonis (NpSRII) results in a slight increase in the lipid dynamics compared to empty DIBMALPs. The light-induced photocycle shows full functionality of DIBMALPs-embedded NpSRII and a significant effect of the protein-to-lipid ratio during preparation on the NpSRII dynamics. This study indicates a possible expansion of the applicability of the DIBMALP technology on studies of membrane protein-protein interaction and oligomerization in a constraining environment.
dc.description.sponsorshipGerman Research Foundation (DFG)German Research Foundation (DFG) [STE640/15, SFB 944/3-2020, TP 26]; RFBRRussian Foundation for Basic Research (RFBR) [18-504-12045]; German Academic Exchange Service (DAAD) and its OstpartnerschaftenprogrammDeutscher Akademischer Austausch Dienst (DAAD); Deutsche Forschungsgemeinschaft (DFG) via the Open Access Publishing Fund of Osnabruck UniversityGerman Research Foundation (DFG); This research was funded by German Research Foundation (DFG, STE640/15) to H.-J.S., (DFG, SFB 944/3-2020, TP 26) to K.C., and RFBR grant no. 18-504-12045 to K.V.S., A.Y.M., K.V.S. and H.-J.S. would like to thank the German Academic Exchange Service (DAAD) and its Ostpartnerschaftenprogramm for support. The authors acknowledge the support from Deutsche Forschungsgemeinschaft (DFG) via the Open Access Publishing Fund of Osnabruck University.
dc.language.isoen
dc.publisherMDPI
dc.relation.ispartofINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
dc.subjectBILAYER PROPERTIES
dc.subjectBiochemistry & Molecular Biology
dc.subjectCHARGED PHOSPHOLIPIDS
dc.subjectChemistry
dc.subjectChemistry, Multidisciplinary
dc.subjectcoarse-grained (CG)
dc.subjectelectron paramagnetic resonance spectroscopy (EPR
dc.subjectESR)
dc.subjectFREE PURIFICATION
dc.subjectHELIX F
dc.subjectlipid nanodiscs
dc.subjectmembrane protein
dc.subjectMEMBRANE-PROTEINS
dc.subjectmolecular dynamics (MD)
dc.subjectNATRONOBACTERIUM-PHARAONIS
dc.subjectNATRONOMONAS-PHARAONIS
dc.subjectnegative-stain and cryo-transmission electron microscopy (EM)
dc.subjectnitroxide spin label
dc.subjectphospholipid bilayer
dc.subjectpolymer
dc.subjectSPIN-LABEL
dc.subjectTRANSDUCER PROTEIN HTRII
dc.subjectVERSATILE TOOL
dc.titleLipid Dynamics in Diisobutylene-Maleic Acid (DIBMA) Lipid Particles in Presence of Sensory Rhodopsin II
dc.typejournal article
dc.identifier.doi10.3390/ijms22052548
dc.identifier.isiISI:000628277200001
dc.description.volume22
dc.description.issue5
dc.contributor.orcid0000-0003-4078-4762
dc.contributor.orcid0000-0002-5888-0157
dc.contributor.orcid0000-0002-3796-3500
dc.contributor.orcid0000-0003-2317-0144
dc.contributor.researcheridV-5214-2017
dc.contributor.researcheridH-3791-2014
dc.identifier.eissn14220067
dc.publisher.placeST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND
dcterms.isPartOf.abbreviationInt. J. Mol. Sci.
dcterms.oaStatusGreen Published, gold
crisitem.author.deptFB 04 - Physik-
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptUniversität Osnabrück-
crisitem.author.deptFB 04 - Physik-
crisitem.author.deptidfb04-
crisitem.author.deptidfb05-
crisitem.author.deptidfb05-
crisitem.author.deptidfb04-
crisitem.author.orcid0000-0003-2317-0144-
crisitem.author.orcid0000-0002-4035-6840-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidVoNa568-
crisitem.author.netidPsOl764-
crisitem.author.netidCoKa893-
crisitem.author.netidShKo001-
crisitem.author.netidStHe633-
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