High-Affinity Labeling and Tracking of Individual Histidine-Tagged Proteins in Live Cells Using Ni2+ Tris-nitrilotriacetic Acid Quantum Dot Conjugates
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Roullier, Victor | |
dc.contributor.author | Clarke, Samuel | |
dc.contributor.author | You, Changjiang | |
dc.contributor.author | Pinaud, Fabien | |
dc.contributor.author | Gouzer, Geraldine | |
dc.contributor.author | Schaible, Dirk | |
dc.contributor.author | Marchi-Artzner, Valerie | |
dc.contributor.author | Piehler, Jacob | |
dc.contributor.author | Dahan, Maxime | |
dc.date.accessioned | 2021-12-23T16:23:05Z | - |
dc.date.available | 2021-12-23T16:23:05Z | - |
dc.date.issued | 2009 | |
dc.identifier.issn | 15306984 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/14410 | - |
dc.description.abstract | Investigation of many cellular processes using fluorescent quantum dots (QDs) is hindered by the nontrivial requirements for OD surface functionalization and targeting. To address these challenges, we designed, characterized and applied QD-trisNTA, which integrates trisnitrilotriacetic acid, a small and high-affinity recognition unit for the ubiquitous polyhistidine protein tag. Using GD-trisNTA, we demonstrate two-color OD tracking of the type-1 interferon receptor subunits in live cells, potentially enabling direct visualization of protein-protein interactions at the single molecule level. | |
dc.language.iso | en | |
dc.publisher | AMER CHEMICAL SOC | |
dc.relation.ispartof | NANO LETTERS | |
dc.subject | BIOTIN LIGASE | |
dc.subject | CDSE/ZNS NANOCRYSTALS | |
dc.subject | Chemistry | |
dc.subject | Chemistry, Multidisciplinary | |
dc.subject | Chemistry, Physical | |
dc.subject | DIFFUSION | |
dc.subject | IMMOBILIZATION | |
dc.subject | LIVING CELLS | |
dc.subject | Materials Science | |
dc.subject | Materials Science, Multidisciplinary | |
dc.subject | MEMBRANE | |
dc.subject | Nanoscience & Nanotechnology | |
dc.subject | PEPTIDES | |
dc.subject | Physics | |
dc.subject | Physics, Applied | |
dc.subject | Physics, Condensed Matter | |
dc.subject | POLYHISTIDINE SEQUENCES | |
dc.subject | RECEPTOR INTERACTIONS | |
dc.subject | Science & Technology - Other Topics | |
dc.subject | SURFACE-PROTEINS | |
dc.title | High-Affinity Labeling and Tracking of Individual Histidine-Tagged Proteins in Live Cells Using Ni2+ Tris-nitrilotriacetic Acid Quantum Dot Conjugates | |
dc.type | journal article | |
dc.identifier.doi | 10.1021/nl9001298 | |
dc.identifier.isi | ISI:000264142100054 | |
dc.description.volume | 9 | |
dc.description.issue | 3 | |
dc.description.startpage | 1228 | |
dc.description.endpage | 1234 | |
dc.contributor.orcid | 0000-0002-7839-6397 | |
dc.contributor.orcid | 0000-0002-4578-6567 | |
dc.contributor.researcherid | K-9256-2013 | |
dc.contributor.researcherid | F-2306-2010 | |
dc.contributor.researcherid | F-1740-2010 | |
dc.contributor.researcherid | L-3901-2014 | |
dc.contributor.researcherid | C-9955-2010 | |
dc.publisher.place | 1155 16TH ST, NW, WASHINGTON, DC 20036 USA | |
dcterms.isPartOf.abbreviation | Nano Lett. | |
crisitem.author.dept | Sonderforschungsbereich 944: Physiologie und Dynamik zellulärer Mikrokompartimente | - |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | organisation19 | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-7839-6397 | - |
crisitem.author.orcid | 0000-0002-2143-2270 | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | YoCh745 | - |
crisitem.author.netid | PiJa938 | - |
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