Oil Body Formation in Marchantia polymorpha Is Controlled by MpC1HDZ and Serves as a Defense against Arthropod Herbivores
DC Element | Wert | Sprache |
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dc.contributor.author | Romani, Facundo | |
dc.contributor.author | Banic, Elizabeta | |
dc.contributor.author | Florent, Stevie N. | |
dc.contributor.author | Kanazawa, Takehiko | |
dc.contributor.author | Goodger, Jason Q. D. | |
dc.contributor.author | Mentink, Remco A. | |
dc.contributor.author | Dierschke, Tom | |
dc.contributor.author | Zachgo, Sabine | |
dc.contributor.author | Ueda, Takashi | |
dc.contributor.author | Bowman, John L. | |
dc.contributor.author | Tsiantis, Miltos | |
dc.contributor.author | Moreno, Javier E. | |
dc.date.accessioned | 2021-12-23T16:23:18Z | - |
dc.date.available | 2021-12-23T16:23:18Z | - |
dc.date.issued | 2020 | |
dc.identifier.issn | 09609822 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/14492 | - |
dc.description.abstract | The origin of a terrestrial flora in the Ordovician required adaptation to novel biotic and abiotic stressors. Oil bodies, a synapomorphy of liverworts, accumulate secondary metabolites, but their function and development arepoorly understood. Oil bodies of Marchantia polymorpha develop within specialized cells as one single large organelle. Here, we show that a class I homeodomain leucine-zipper (C1HDZ) transcription factor controls the differentiation of oil body cells in two different ecotypes of the liverwort M. polymorpha, a model genetic system for early divergent land plants. In flowering plants, these transcription factors primarily modulate responses to abiotic stress, including drought. However, loss-of-function alleles of the single ortholog gene, MpC1HDZ, in M. polymorpha did not exhibit phenotypes associated with abiotic stress. Rather, Mpc1hdz mutant plants were more susceptible to herbivory, and total plant extracts of the mutant exhibited reduced antibacterial activity. Transcriptomic analysis of the mutant revealed a reduction in expression of genes related to secondary metabolism that was accompanied by a specific depletion of oil body terpenoid compounds. Through time-lapse imaging, we observed that MpC1HDZ expression maxima precede oil body formation, indicating that MpC1HDZ mediates differentiation of oil body cells. Our results indicate that M. polymorpha oil bodies, and MpC1HDZ, are critical for defense against herbivory, but not for abiotic stress tolerance. Thus, C1HDZ genes were co-opted to regulate separate responses to biotic and abiotic stressors in two distinct land plant lineages. | |
dc.description.sponsorship | Agencia I+D+i [PICT2017-1484, PICT2018-00650]; CONICETConsejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET) [PIP267]; Company of Biologists; Australian Research CouncilAustralian Research Council [DP170100049]; Ministry of Education, Culture, Sports, Science and Technology of JapanMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT) [17K19412, 18H02470, 19H05675, 17H07333, 18K14738]; Mitsubishi Foundation; Yamada Science Foundation; Max Planck SocietyMax Planck SocietyFoundation CELLEX; Deutsche Forschungsgemeinschaft MAdLandGerman Research Foundation (DFG) [SPP TS 229/4-1]; Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [SFB 944]; We thank Dr. Teruyuki Niimi (National Institute for Basic Biology) and Dr. Takahisa Miyatake (Okayama University) for identification of Armadillidium vulgare. The support of plant cultivation rooms was provided by the Model Plant Research Facility of National Institute for Basic Biology. We thank Dr. Magnus Eklund (Uppsala University) for his help with transcriptional reporters, Santiago Fari ` as (UNL) for plant pictures, Dr. Feng Cheng (University of Tennessee) for helpful comments, Dr. Kimitsune Ishizaki (Kobe University) for the cloning vectors, and Dr. Keisuke Inoue for providing pMpGE_En04 and pBC-14. We thank Dr. Saiko Yoshida and Dr. Peter Huijser for assistance with confocal imaging and image analysis, Michelle van der Gragt for GUS stainings of BoGa plants, and Dr. Hirofumi Nagakami for helpful discussions. This work was supported by Agencia I+D+i (PICT2017-1484 and PICT2018-00650 to J.E.M.) and CONICET (PIP267 to J.E.M.). F.R. is a doctoral fellow of CONICET. F.R. received a Travelling Fellowship from The Company of Biologists. J.E.M. is a CONICET career member. S.N.F. and J.L.B. were supported by the Australian Research Council (DP170100049). T.U. and T.K. were financially supported by Grantsin-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (to T.U.: 17K19412, 18H02470, and19H05675; to T.K.: 17H07333 and 18K14738), The Mitsubishi Foundation, and Yamada Science Foundation. M.T. was supported by a core grant from the Max Planck Society and the Deutsche Forschungsgemeinschaft MAdLand SPP TS 229/4-1. S.Z. acknowledges support from the Deutsche Forschungsgemeinschaft (SFB 944). | |
dc.language.iso | en | |
dc.publisher | CELL PRESS | |
dc.relation.ispartof | CURRENT BIOLOGY | |
dc.subject | AGROBACTERIUM-MEDIATED TRANSFORMATION | |
dc.subject | ARABIDOPSIS | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | Biology | |
dc.subject | BIOSYNTHESIS | |
dc.subject | Cell Biology | |
dc.subject | EVOLUTION | |
dc.subject | I GENE | |
dc.subject | LAND PLANTS | |
dc.subject | Life Sciences & Biomedicine - Other Topics | |
dc.subject | LIVERWORTS | |
dc.subject | LOCALIZATION | |
dc.subject | TOLERANCE | |
dc.subject | WATER-DEFICIT | |
dc.title | Oil Body Formation in Marchantia polymorpha Is Controlled by MpC1HDZ and Serves as a Defense against Arthropod Herbivores | |
dc.type | journal article | |
dc.identifier.doi | 10.1016/j.cub.2020.05.081 | |
dc.identifier.isi | ISI:000590260800030 | |
dc.description.volume | 30 | |
dc.description.issue | 14 | |
dc.description.startpage | 2815+ | |
dc.contributor.orcid | 0000-0003-3954-6740 | |
dc.contributor.orcid | 0000-0002-5190-892X | |
dc.contributor.orcid | 0000-0003-0472-2273 | |
dc.contributor.orcid | 0000-0001-6559-6202 | |
dc.contributor.orcid | 0000-0002-3547-5765 | |
dc.contributor.orcid | 0000-0001-7392-3891 | |
dc.contributor.orcid | 0000-0001-7347-3691 | |
dc.contributor.researcherid | AAS-1310-2020 | |
dc.contributor.researcherid | G-6984-2011 | |
dc.contributor.researcherid | U-8023-2017 | |
dc.identifier.eissn | 18790445 | |
dc.publisher.place | 50 HAMPSHIRE ST, FLOOR 5, CAMBRIDGE, MA 02139 USA | |
dcterms.isPartOf.abbreviation | Curr. Biol. | |
dcterms.oaStatus | Green Submitted, Bronze | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | ZaSa518 | - |
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