DC Field | Value | Language |
dc.contributor.author | Kramer, Manuela | |
dc.contributor.author | Rodriguez-Heredia, Melvin | |
dc.contributor.author | Saccon, Francesco | |
dc.contributor.author | Mosebach, Laura | |
dc.contributor.author | Twachtmann, Manuel | |
dc.contributor.author | Krieger-Liszkay, Anja | |
dc.contributor.author | Duffy, Chris | |
dc.contributor.author | Knell, Robert J. | |
dc.contributor.author | Finazzi, Giovanni | |
dc.contributor.author | Hanke, Guy Thomas | |
dc.date.accessioned | 2021-12-23T16:23:38Z | - |
dc.date.available | 2021-12-23T16:23:38Z | - |
dc.date.issued | 2021 | |
dc.identifier.issn | 2050084X | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/14610 | - |
dc.description.abstract | During photosynthesis, electron transport is necessary for carbon assimilation and must be regulated to minimize free radical damage. There is a longstanding controversy over the role of a critical enzyme in this process (ferredoxin:NADP(H) oxidoreductase, or FNR), and in particular its location within chloroplasts. Here we use immunogold labelling to prove that FNR previously assigned as soluble is in fact membrane associated. We combined this technique with a genetic approach in the model plant Arabidopsis to show that the distribution of this enzyme between different membrane regions depends on its interaction with specific tether proteins. We further demonstrate a correlation between the interaction of FNR with different proteins and the activity of alternative photosynthetic electron transport pathways. This supports a role for FNR location in regulating photosynthetic electron flow during the transition from dark to light. | |
dc.description.sponsorship | Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [SFB944]; Biotechnology and Biological Sciences Research CouncilUK Research & Innovation (UKRI)Biotechnology and Biological Sciences Research Council (BBSRC) [BB/R004838/1]; University Grenoble Alps [ANR-10-LABX-49-01]; H2020 European Research Council [833184]; LabEx Saclay Plant Sciences SPS [ANR-10-LABX-0040-SPS]; French Infrastructure for Integrated Structural Biology [ANR-10-INSB-05]; Bayer CropScienceBayer AG [F-2016-BS-0555]; University Grenoble Alps CBH-EUR-GS [ANR-17-EURE-0003]; Deutsche Forschungsgemeinschaft SFB944 project 2 Manuela Kramer Guy Thomas Hanke; Biotechnology and Biological Sciences Research Council BB/R004838/1 Guy Thomas Hanke; University Grenoble Alps ANR-10-LABX-49-01 Giovanni Finazzi; H2020 European Research Council 833184 Giovanni Finazzi; LabEx Saclay Plant SciencesSPS ANR-10-LABX-0040-SPS Anja Krieger-Liszkay; French Infrastructure for Integrated Structural Biology ANR-10-INSB-05 Anja Krieger-Liszkay; Bayer CropScience F-2016-BS-0555 Manuela Kramer; University Grenoble Alps CBH-EUR-GS (ANR-17-EURE-0003) Giovanni Finazzi; The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. | |
dc.language.iso | en | |
dc.publisher | eLIFE SCIENCES PUBL LTD | |
dc.relation.ispartof | ELIFE | |
dc.subject | Biology | |
dc.subject | C-TERMINAL TYROSINE | |
dc.subject | CHLAMYDOMONAS-REINHARDTII | |
dc.subject | CYCLIC PHOTOPHOSPHORYLATION | |
dc.subject | FERREDOXIN-NADP(+) OXIDOREDUCTASE | |
dc.subject | GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE | |
dc.subject | Life Sciences & Biomedicine - Other Topics | |
dc.subject | NADP+ OXIDOREDUCTASE | |
dc.subject | PHOTOSYSTEM-I | |
dc.subject | PLASTOQUINONE REDUCTASE | |
dc.subject | PLUS OXIDOREDUCTASE | |
dc.subject | THYLAKOID MEMBRANES | |
dc.title | Regulation of photosynthetic electron flow on dark to light transition by ferredoxin:NADP(H) oxidoreductase interactions | |
dc.type | journal article | |
dc.identifier.doi | 10.7554/eLife.56088 | |
dc.identifier.isi | ISI:000636043500001 | |
dc.description.volume | 10 | |
dc.contributor.orcid | 0000-0002-6167-926X | |
dc.contributor.orcid | 0000-0001-7141-4129 | |
dc.publisher.place | SHERATON HOUSE, CASTLE PARK, CAMBRIDGE, CB3 0AX, ENGLAND | |
dcterms.isPartOf.abbreviation | eLife | |
dcterms.oaStatus | Green Published, gold, Green Submitted | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | HaGu059 | - |