A physical model describing the interaction of nuclear transport receptors with FG nucleoporin domain assemblies
DC Element | Wert | Sprache |
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dc.contributor.author | Zahn, Raphael | |
dc.contributor.author | Osmanovic, Dino | |
dc.contributor.author | Ehret, Severin | |
dc.contributor.author | Callis, Carolina Araya | |
dc.contributor.author | Frey, Steffen | |
dc.contributor.author | Stewart, Murray | |
dc.contributor.author | You, Changjiang | |
dc.contributor.author | Goerlich, Dirk | |
dc.contributor.author | Hoogenboom, Bart W. | |
dc.contributor.author | Richter, Ralf P. | |
dc.date.accessioned | 2021-12-23T16:23:43Z | - |
dc.date.available | 2021-12-23T16:23:43Z | - |
dc.date.issued | 2016 | |
dc.identifier.issn | 2050084X | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/14635 | - |
dc.description.abstract | The permeability barrier of nuclear pore complexes (NPCs) controls bulk nucleocytoplasmic exchange. It consists of nucleoporin domains rich in phenylalanine-glycine motifs (FG domains). As a bottom-up nanoscale model for the permeability barrier, we have used planar films produced with three different end-grafted FG domains, and quantitatively analyzed the binding of two different nuclear transport receptors (NTRs), NTF2 and Importin beta, together with the concomitant film thickness changes. NTR binding caused only moderate changes in film thickness; the binding isotherms showed negative cooperativity and could all be mapped onto a single master curve. This universal NTR binding behavior a key element for the transport selectivity of the NPC was quantitatively reproduced by a physical model that treats FG domains as regular, flexible polymers, and NTRs as spherical colloids with a homogeneous surface, ignoring the detailed arrangement of interaction sites along FG domains and on the NTR surface. | |
dc.description.sponsorship | European CommissionEuropean CommissionEuropean Commission Joint Research Centre [FP7-PEOPLE-2012-IEF-327975]; European Research CouncilEuropean Research Council (ERC)European Commission [FP7-ERC-2012-StG-306435]; Dr. Mortimer and Theresa Sackler Foundation; Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [SFB 944]; Medical Research CouncilUK Research & Innovation (UKRI)Medical Research Council UK (MRC)European Commission [U105178939]; Spanish Ministry for Economy and CompetitivenessSpanish Government [MAT2011-24306]; MRCUK Research & Innovation (UKRI)Medical Research Council UK (MRC) [MC_U105178939] Funding Source: UKRI; Medical Research CouncilUK Research & Innovation (UKRI)Medical Research Council UK (MRC)European Commission [MC_U105178939] Funding Source: researchfish; European Commission FP7-PEOPLE-2012-IEF-327975 Raphael Zahn; European Research Council FP7-ERC-2012-StG-306435 Ralf P Richter; Dr. Mortimer and Theresa Sackler Foundation Dino Osmanovic Bart W Hoogenboom; Deutsche Forschungsgemeinschaft SFB 944 Changjiang You; Medical Research Council Grant U105178939 Murray Stewart; Spanish Ministry for Economy and Competitiveness MAT2011-24306 Ralf P Richter | |
dc.language.iso | en | |
dc.publisher | ELIFE SCIENCES PUBLICATIONS LTD | |
dc.relation.ispartof | ELIFE | |
dc.subject | BINDING | |
dc.subject | Biology | |
dc.subject | IMPORTIN-BETA | |
dc.subject | KINETIC-ANALYSIS | |
dc.subject | Life Sciences & Biomedicine - Other Topics | |
dc.subject | MOLECULAR-DYNAMICS | |
dc.subject | NUCLEOCYTOPLASMIC TRANSPORT | |
dc.subject | PERMEABILITY | |
dc.subject | PORE COMPLEX | |
dc.subject | PROTEINS | |
dc.subject | REPEAT REGIONS | |
dc.subject | STRUCTURAL-ANALYSIS | |
dc.title | A physical model describing the interaction of nuclear transport receptors with FG nucleoporin domain assemblies | |
dc.type | journal article | |
dc.identifier.doi | 10.7554/eLife.14119 | |
dc.identifier.isi | ISI:000376882000001 | |
dc.description.volume | 5 | |
dc.contributor.orcid | 0000-0003-0223-0697 | |
dc.contributor.orcid | 0000-0002-7839-6397 | |
dc.contributor.orcid | 0000-0003-3071-2837 | |
dc.contributor.orcid | 0000-0001-7792-2247 | |
dc.contributor.orcid | 0000-0001-7690-0660 | |
dc.contributor.orcid | 0000-0002-8882-4324 | |
dc.contributor.orcid | 0000-0002-5134-9961 | |
dc.contributor.orcid | 0000-0002-5818-6966 | |
dc.contributor.researcherid | M-3706-2013 | |
dc.contributor.researcherid | L-3901-2014 | |
dc.contributor.researcherid | B-3343-2013 | |
dc.contributor.researcherid | AAR-5457-2021 | |
dc.contributor.researcherid | J-9136-2014 | |
dc.publisher.place | SHERATON HOUSE, CASTLE PARK, CAMBRIDGE, CB3 0AX, ENGLAND | |
dcterms.isPartOf.abbreviation | eLife | |
dcterms.oaStatus | Green Submitted, Green Published, gold | |
crisitem.author.dept | Sonderforschungsbereich 944: Physiologie und Dynamik zellulärer Mikrokompartimente | - |
crisitem.author.deptid | organisation19 | - |
crisitem.author.orcid | 0000-0002-7839-6397 | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | YoCh745 | - |
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