Non-specific interactions govern cytosolic diffusion of nanosized objects in mammalian cells
DC Element | Wert | Sprache |
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dc.contributor.author | Etoc, Fred | |
dc.contributor.author | Balloul, Elie | |
dc.contributor.author | Vicario, Chiara | |
dc.contributor.author | Normanno, Davide | |
dc.contributor.author | Lisse, Domenik | |
dc.contributor.author | Sittner, Assa | |
dc.contributor.author | Piehler, Jacob | |
dc.contributor.author | Dahan, Maxime | |
dc.contributor.author | Coppey, Mathieu | |
dc.date.accessioned | 2021-12-23T16:23:53Z | - |
dc.date.available | 2021-12-23T16:23:53Z | - |
dc.date.issued | 2018 | |
dc.identifier.issn | 14761122 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/14700 | - |
dc.description.abstract | The diffusivity of macromolecules in the cytoplasm of eukaryotic cells varies over orders of magnitude and dictates the kinetics of cellular processes. However, a general description that associates the Brownian or anomalous nature of intracellular diffusion to the architectural and biochemical properties of the cytoplasm has not been achieved. Here we measure the mobility of individual fluorescent nanoparticles in living mammalian cells to obtain a comprehensive analysis of cytoplasmic diffusion. We identify a correlation between tracer size, its biochemical nature and its mobility. Inert particles with size equal or below 50 nm behave as Brownian particles diffusing in a medium of low viscosity with negligible effects of molecular crowding. Increasing the strength of non-specific interactions of the nanoparticles within the cytoplasm gradually reduces their mobility and leads to subdiffusive behaviour. These experimental observations and the transition from Brownian to subdiffusive motion can be captured in a minimal phenomenological model. | |
dc.description.sponsorship | Ecole Doctorale Frontieres du Vivant (FdV)-Programme Bettencourt; French National Research Agency (ANR) Paris-Science-Lettres ProgramFrench National Research Agency (ANR) [ANR-10-IDEX-0001-02 PSL]; Labex CelTisPhyBio [ANR-10-LBX-0038]; Human Frontier Science ProgramHuman Frontier Science Program [RGP0005/2007]; ANRFrench National Research Agency (ANR) [ANR-10-INSB-04]; European Union's Horizon 2020 Research and Innovation Programme [686841]; We acknowledge M. Coppey-Moisan, B. Goud and S. Letard and P. Dubreuil for supplying various cells (see text). E.B. acknowledges the Ecole Doctorale Frontieres du Vivant (FdV)-Programme Bettencourt for financial support. M.D. and M.C. acknowledge financial support from the French National Research Agency (ANR) Paris-Science-Lettres Program (ANR-10-IDEX-0001-02 PSL), Labex CelTisPhyBio (ANR-10-LBX-0038), the Human Frontier Science Program (grant no. RGP0005/2007) and the France-Biolmaging infrastructure supported by ANR Grant ANR-10-INSB-04 (Investments for the Future). This project has received funding from the European Union's Horizon 2020 Research and Innovation Programme under grant agreement no. 686841 (MAGNEURON). | |
dc.language.iso | en | |
dc.publisher | NATURE PUBLISHING GROUP | |
dc.relation.ispartof | NATURE MATERIALS | |
dc.subject | ACTIN | |
dc.subject | ANOMALOUS DIFFUSION | |
dc.subject | Chemistry | |
dc.subject | Chemistry, Physical | |
dc.subject | CYTOPLASM | |
dc.subject | MACROMOLECULES | |
dc.subject | Materials Science | |
dc.subject | Materials Science, Multidisciplinary | |
dc.subject | MOLECULES | |
dc.subject | PARTICLES | |
dc.subject | Physics | |
dc.subject | Physics, Applied | |
dc.subject | Physics, Condensed Matter | |
dc.subject | PROTEINS | |
dc.subject | QUANTUM DOTS | |
dc.subject | TRACKING | |
dc.subject | VISCOSITY | |
dc.title | Non-specific interactions govern cytosolic diffusion of nanosized objects in mammalian cells | |
dc.type | journal article | |
dc.identifier.doi | 10.1038/s41563-018-0120-7 | |
dc.identifier.isi | ISI:000439573400020 | |
dc.description.volume | 17 | |
dc.description.issue | 8 | |
dc.description.startpage | 740+ | |
dc.contributor.orcid | 0000-0001-8924-3233 | |
dc.contributor.orcid | 0000-0003-4740-5542 | |
dc.contributor.orcid | 0000-0002-1633-8191 | |
dc.contributor.researcherid | AAR-3207-2020 | |
dc.identifier.eissn | 14764660 | |
dc.publisher.place | MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND | |
dcterms.isPartOf.abbreviation | Nat. Mater. | |
dcterms.oaStatus | Green Submitted | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-2143-2270 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | PiJa938 | - |
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geprüft am 12.05.2024