DC Field | Value | Language |
dc.contributor.author | Lin, Jiangguo | |
dc.contributor.author | Countryman, Preston | |
dc.contributor.author | Chen, Haijiang | |
dc.contributor.author | Pan, Hai | |
dc.contributor.author | Fan, Yanlin | |
dc.contributor.author | Jiang, Yunyun | |
dc.contributor.author | Kaur, Parminder | |
dc.contributor.author | Miao, Wang | |
dc.contributor.author | Gurgel, Gisele | |
dc.contributor.author | You, Changjiang | |
dc.contributor.author | Piehler, Jacob | |
dc.contributor.author | Kad, Neil M. | |
dc.contributor.author | Riehn, Robert | |
dc.contributor.author | Opresko, Patricia L. | |
dc.contributor.author | Smith, Susan | |
dc.contributor.author | Tao, Yizhi Jane | |
dc.contributor.author | Wang, Hong | |
dc.date.accessioned | 2021-12-23T16:23:54Z | - |
dc.date.available | 2021-12-23T16:23:54Z | - |
dc.date.issued | 2016 | |
dc.identifier.issn | 03051048 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/14705 | - |
dc.description.abstract | Proper chromosome alignment and segregation during mitosis depend on cohesion between sister chromatids. Cohesion is thought to occur through the entrapment of DNA within the tripartite ring (Smc1, Smc3 and Rad21) with enforcement from a fourth subunit (SA1/SA2). Surprisingly, cohesin rings do not play a major role in sister telomere cohesion. Instead, this role is replaced by SA1 and telomere binding proteins (TRF1 and TIN2). Neither the DNA binding property of SA1 nor this unique telomere cohesion mechanism is understood. Here, using single-molecule fluorescence imaging, we discover that SA1 displays two-state binding on DNA: searching by one-dimensional (1D) free diffusion versus recognition through subdiffusive sliding at telomeric regions. The AT-hook motif in SA1 plays dual roles in modulating non-specific DNA binding and subdiffusive dynamics over telomeric regions. TRF1 tethers SA1 within telomeric regions that SA1 transiently interacts with. SA1 and TRF1 together form longer DNA-DNA pairing tracts than with TRF1 alone, as revealed by atomic force microscopy imaging. These results suggest that at telomeres cohesion relies on the molecular interplay between TRF1 and SA1 to promote DNA-DNA pairing, while along chromosomal arms the core cohesin assembly might also depend on SA1 1D diffusion on DNA and sequence-specific DNA binding. | |
dc.description.sponsorship | National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01ES022944, R00ES016758, R01GM107559, R01CA116352, P30 ES025128]; Welch FoundationThe Welch Foundation [C-1565]; China Scholarship CouncilChina Scholarship Council [201206320020]; NIGMSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [R01GM107559]; BBSRCUK Research & Innovation (UKRI)Biotechnology and Biological Sciences Research Council (BBSRC) [BB/I003460/1] Funding Source: UKRI; Biotechnology and Biological Sciences Research CouncilUK Research & Innovation (UKRI)Biotechnology and Biological Sciences Research Council (BBSRC) [BB/I003460/1] Funding Source: researchfish; NATIONAL CANCER INSTITUTEUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Cancer Institute (NCI) [R01CA116352] Funding Source: NIH RePORTER; NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Environmental Health Sciences (NIEHS) [R00ES016758, P30ES025128, R01ES022944] Funding Source: NIH RePORTER; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [R01GM107559] Funding Source: NIH RePORTER; National Institutes of Health [R01ES022944 to P.L.O., R00ES016758 to H.W., R01GM107559 to R.R., H.W., R01CA116352 to S.S., P30 ES025128 to CHHE at NCSU]; Welch Foundation [C-1565 to Y.J.T.]; China Scholarship Council [201206320020 to H.C.]. Funding for open access charge: NIGMS [R01GM107559]. | |
dc.language.iso | en | |
dc.publisher | OXFORD UNIV PRESS | |
dc.relation.ispartof | NUCLEIC ACIDS RESEARCH | |
dc.subject | AT-HOOK | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | DIFFUSION-DRIVEN MECHANISMS | |
dc.subject | GENE-EXPRESSION | |
dc.subject | MAMMALIAN TELOMERES | |
dc.subject | NUCLEIC-ACIDS | |
dc.subject | NUCLEOTIDE EXCISION-REPAIR | |
dc.subject | ONE-DIMENSIONAL DIFFUSION | |
dc.subject | PROTEIN TRANSLOCATION | |
dc.subject | REPRESSOR-OPERATOR INTERACTION | |
dc.subject | SISTER CHROMATIDS | |
dc.title | Functional interplay between SA1 and TRF1 in telomeric DNA binding and DNA-DNA pairing | |
dc.type | journal article | |
dc.identifier.doi | 10.1093/nar/gkw518 | |
dc.identifier.isi | ISI:000382999300033 | |
dc.description.volume | 44 | |
dc.description.issue | 13 | |
dc.description.startpage | 6363 | |
dc.description.endpage | 6376 | |
dc.contributor.orcid | 0000-0002-4408-3560 | |
dc.contributor.orcid | 0000-0002-7839-6397 | |
dc.contributor.orcid | 0000-0003-0165-3559 | |
dc.contributor.orcid | 0000-0001-9183-4315 | |
dc.contributor.orcid | 0000-0002-8213-5915 | |
dc.contributor.orcid | 0000-0002-3491-8595 | |
dc.contributor.researcherid | A-7195-2011 | |
dc.contributor.researcherid | L-3901-2014 | |
dc.contributor.researcherid | D-1893-2009 | |
dc.contributor.researcherid | F-3164-2014 | |
dc.identifier.eissn | 13624962 | |
dc.publisher.place | GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND | |
dcterms.isPartOf.abbreviation | Nucleic Acids Res. | |
dcterms.oaStatus | Green Accepted, Green Submitted, Green Published, gold | |
crisitem.author.dept | Sonderforschungsbereich 944: Physiologie und Dynamik zellulärer Mikrokompartimente | - |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | organisation19 | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-7839-6397 | - |
crisitem.author.orcid | 0000-0002-2143-2270 | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | YoCh745 | - |
crisitem.author.netid | PiJa938 | - |