Mice lacking lipid droplet-associated hydrolase, a gene linked to human prostate cancer, have normal cholesterol ester metabolism
DC Element | Wert | Sprache |
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dc.contributor.author | Kory, Nora | |
dc.contributor.author | Grond, Susanne | |
dc.contributor.author | Kamat, Siddhesh S. | |
dc.contributor.author | Li, Zhihuan | |
dc.contributor.author | Krahmer, Natalie | |
dc.contributor.author | Chitraju, Chandramohan | |
dc.contributor.author | Zhou, Ping | |
dc.contributor.author | Frohlich, Florian | |
dc.contributor.author | Semova, Ivana | |
dc.contributor.author | Ejsing, Christer | |
dc.contributor.author | Zechner, Rudolf | |
dc.contributor.author | Cravatt, Benjamin F. | |
dc.contributor.author | Farese, Jr., Robert V. | |
dc.contributor.author | Walther, Tobias C. | |
dc.date.accessioned | 2021-12-23T16:24:02Z | - |
dc.date.available | 2021-12-23T16:24:02Z | - |
dc.date.issued | 2017 | |
dc.identifier.issn | 00222275 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/14755 | - |
dc.description.abstract | Variations in the gene LDAH (C2ORF43), which encodes lipid droplet-associated hydrolase (LDAH), are among few loci associated with human prostate cancer. Homologs of LDAH have been identified as proteins of lipid droplets (LDs). LDs are cellular organelles that store neutral lipids, such as triacylglycerols and sterol esters, as precursors for membrane components and as reservoirs of metabolic energy. LDAH is reported to hydrolyze cholesterol esters and to be important in macrophage cholesterol ester metabolism. Here, we confirm that LDAH is localized to LDs in several model systems. We generated a murine model in which Ldah is disrupted but found no evidence for a major function of LDAH in cholesterol ester or triacylglycerol metabolism in vivo, nor a role in energy or glucose metabolism.(Jlr) Our data suggest that LDAH is not a major cholesterol ester hydrolase, and an alternative metabolic function may be responsible for its possible effect on development of prostate cancer. | |
dc.description.sponsorship | National Institues of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [1R01GM097194, R01DK101579]; G. Harold and Leila Y. Mathers Foundation; J. David Gladstone Institute; American Heart AssociationAmerican Heart Association; National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [U01HG004085, DK059637, DK020593, DK059635]; CSD Consortium [U01HG004080]; Austrian Science Fund (FWF)Austrian Science Fund (FWF) [W 901, F 3002] Funding Source: researchfish; NATIONAL CENTER FOR RESEARCH RESOURCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Center for Research Resources (NCRR) [U42RR024244] Funding Source: NIH RePORTER; NATIONAL HUMAN GENOME RESEARCH INSTITUTEUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Human Genome Research Institute (NHGRI) [U01HG004085, U01HG004080] Funding Source: NIH RePORTER; NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) [P60DK020593, P30DK020593, R01DK101579, U24DK059637, U24DK059635] Funding Source: NIH RePORTER; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [R01GM097194] Funding Source: NIH RePORTER; This work was supported by the National Institues of Health Grant 1R01GM097194 (to T.C.W.), Grant R01DK101579 (to R.V.F.), the G. Harold and Leila Y. Mathers Foundation (to T.C.W.), and J. David Gladstone Institute (to R.V.F.). N.K. was supported by an American Heart Association predoctoral fellowship. National Institutes of Health grants to Velocigene at Regeneron Inc. (U01HG004085) and the CSD Consortium (U01HG004080) funded the generation of gene-targeted embryonic stem cells for 8,500 genes in the Knockout Mouse Project (KOMP) and archived and distributed by the KOMP Repository at the University of California, Davis, and Children's Hospital Oakland Research Institute (U42RR024244). Testosterone and corticosterone assays were performed by the Vanderbilt University Medical Center Hormone Assay and Analytical Services Core, which is supported by National Institutes of Health Grants DK059637 and DK020593. T.C.W is an investigator of the Howard Hughes Medical Institute. Dr. Michael Jurczak and the Yale Mouse Metabolic Phenotyping Center were funded by National Institutes of Health Grant DK059635. The content is solely the responsibility of the authors and do not necessarily represent the official views of the National Institutes of Health. | |
dc.language.iso | en | |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | |
dc.relation.ispartof | JOURNAL OF LIPID RESEARCH | |
dc.subject | ACCUMULATION | |
dc.subject | ACTIVATION | |
dc.subject | animal models | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | BREAST | |
dc.subject | cholesterol efflux | |
dc.subject | ENZYMES | |
dc.subject | EXPRESSION | |
dc.subject | HORMONE-SENSITIVE LIPASE | |
dc.subject | lipase | |
dc.subject | lipoprotein metabolism | |
dc.subject | LOCI | |
dc.subject | PROTEINS | |
dc.subject | RISK VARIANTS | |
dc.subject | triglycerides | |
dc.title | Mice lacking lipid droplet-associated hydrolase, a gene linked to human prostate cancer, have normal cholesterol ester metabolism | |
dc.type | journal article | |
dc.identifier.doi | 10.1194/jlr.M072538 | |
dc.identifier.isi | ISI:000392408700019 | |
dc.description.volume | 58 | |
dc.description.issue | 1 | |
dc.description.startpage | 226 | |
dc.description.endpage | 235 | |
dc.contributor.orcid | 0000-0001-5307-9021 | |
dc.contributor.orcid | 0000-0003-4063-7367 | |
dc.contributor.orcid | 0000-0001-8338-8976 | |
dc.contributor.orcid | 0000-0003-4963-0276 | |
dc.contributor.orcid | 0000-0001-5483-1182 | |
dc.contributor.orcid | 0000-0002-7121-9013 | |
dc.contributor.orcid | 0000-0001-8307-2189 | |
dc.contributor.orcid | 0000-0001-6132-7574 | |
dc.contributor.orcid | 0000-0003-1442-1327 | |
dc.contributor.researcherid | AAL-9467-2020 | |
dc.contributor.researcherid | Q-6733-2019 | |
dc.contributor.researcherid | AAL-3392-2020 | |
dc.contributor.researcherid | O-8188-2018 | |
dc.identifier.eissn | 15397262 | |
dc.publisher.place | 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA | |
dcterms.isPartOf.abbreviation | J. Lipid Res. | |
dcterms.oaStatus | Green Published, hybrid | |
crisitem.author.dept | Sonderforschungsbereich 944: Physiologie und Dynamik zellulärer Mikrokompartimente | - |
crisitem.author.deptid | organisation19 | - |
crisitem.author.orcid | 0000-0001-8307-2189 | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | FrFl166 | - |
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