DC Element | Wert | Sprache |
dc.contributor.author | Seela, F. | |
dc.contributor.author | Rosemeyer, H. | |
dc.contributor.author | Krecmerova, M. | |
dc.contributor.author | Röling, A. | |
dc.date.accessioned | 2021-12-23T16:26:22Z | - |
dc.date.available | 2021-12-23T16:26:22Z | - |
dc.date.issued | 1991 | |
dc.identifier.issn | 02613166 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/14952 | - |
dc.description.abstract | 1-(2'-Deoxy-beta-D-threo-pentofuranosyl)thymine (xTd) and -adenine (xAd) were converted into their appropriately protected 3'-phosphonates 1a, 2a as well as their 2-cyanoethyl phosphoramidites 1b, 2b. These compounds were used for solid-phase syntheses of the oligo(2'-deoxy-beta-D-xylonucleotides) 5-8. Structural properties and behavior against nucleases is described. Apart from oligo(2'-deoxyxylonucleotides) the PCR-amplification of a pUC18 DNA fragment with Taq polymerase was studied in the presence of the 7-deazapurine derivatives of dGTP, dATP, and dITP. The incorporation efficiency of the modified compounds was compared with those of the parent nucleotides. 7-Deaza-2'-deoxyguanosine protected the DNA-fragment from hydrolysis by the restriction endodeoxyribonuclease Eco RI, Pst I, Bam HI, and Sma I if the nucleoside was located within the recognition site. | |
dc.language.iso | en | |
dc.relation.ispartof | Nucleic acids symposium series | |
dc.subject | adenosine, 58-61-7 | |
dc.subject | DNA, 9007-49-2 | |
dc.subject | DNA directed DNA polymerase, 9012-90-2 | |
dc.subject | thymidine, 50-89-5 | |
dc.subject | xylose, 25990-60-7, 58-86-6 | |
dc.subject | 1-(2'-deoxy-beta-threopentofuranosyl)adenine, 13276-53-4, 58-61-7 | |
dc.subject | 1-(2'-deoxy-beta-threopentofuranosyl)thymine, 16053-52-4 | |
dc.subject | Adenosine, 58-61-7 | |
dc.subject | DNA, 9007-49-2 | |
dc.subject | DNA Restriction Enzymes, EC 3.1.21.-, 58-61-7 | |
dc.subject | DNA-Directed DNA Polymerase, EC 2.7.7.7 | |
dc.subject | Oligonucleotides | |
dc.subject | Phosphonic Acids | |
dc.subject | Taq Polymerase, EC 2.7.7.- | |
dc.subject | Thymidine, 50-89-5, 58-61-7 | |
dc.subject | Xylose | |
dc.subject | 1 (2' deoxy beta threopentofuranosyl)adenine | |
dc.subject | 1 (2' deoxy beta threopentofuranosyl)thymine | |
dc.subject | 1-(2'-deoxy-beta-threopentofuranosyl)adenine | |
dc.subject | 1-(2'-deoxy-beta-threopentofuranosyl)thymine | |
dc.subject | adenosine | |
dc.subject | DNA | |
dc.subject | DNA directed DNA polymerase | |
dc.subject | drug derivative | |
dc.subject | oligonucleotide | |
dc.subject | phosphonic acid derivative | |
dc.subject | restriction endonuclease | |
dc.subject | Taq polymerase | |
dc.subject | thymidine | |
dc.subject | xylose, article | |
dc.subject | chemical structure | |
dc.subject | chemistry | |
dc.subject | enzyme specificity | |
dc.subject | metabolism | |
dc.subject | methodology | |
dc.subject | molecular genetics | |
dc.subject | nucleotide sequence | |
dc.subject | polymerase chain reaction | |
dc.subject | restriction mapping | |
dc.subject | synthesis, Adenosine | |
dc.subject | Base Sequence | |
dc.subject | DNA | |
dc.subject | DNA Restriction Enzymes | |
dc.subject | DNA-Directed DNA Polymerase | |
dc.subject | Molecular Sequence Data | |
dc.subject | Molecular Structure | |
dc.subject | Oligonucleotides | |
dc.subject | Phosphonic Acids | |
dc.subject | Polymerase Chain Reaction | |
dc.subject | Restriction Mapping | |
dc.subject | Substrate Specificity | |
dc.subject | Taq Polymerase | |
dc.subject | Thymidine | |
dc.subject | Xylose | |
dc.title | Solid-phase synthesis of oligo(2'-deoxyxylonucleotides) and PCR amplification of base-modified DNA fragments. | |
dc.type | journal article | |
dc.identifier.pmid | 1668697 | |
dc.identifier.scopus | 2-s2.0-0026289224 | |
dc.identifier.url | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026289224&partnerID=40&md5=9b8b0e72c8f2dc290f59f4b300e21675 | |
dc.description.issue | 24 | |
dc.description.startpage | 87 | |
dc.description.endpage | 90 | |
dcterms.isPartOf.abbreviation | Nucleic Acids Symp. Ser. | |
crisitem.author.dept | Institut für Chemie neuer Materialien | - |
crisitem.author.deptid | institute11 | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | RoHe783 | - |