SERS microscopy: Plasmonic nanoparticle probes and biomedical applications

Autor(en): Gellner, M.
Schütz, M.
Salehi, M.
Packeisen, J.
Ströbel, P.
Marx, A.
Schmuck, C.
Schlücker, S.
Stichwörter: Antibodies; Bio-conjugation; Biomedical applications; Biomedical imaging applications; Chemical detection; Detection scheme; Gold; Laser excitation; Medical imaging; Metal core; Microscopy; Molecules; Nanoparticle probes; Nanoparticles; Nanoshell; Nanoshells; Nanostructured materials; Organic polymers; Particle aggregation; Plasmonic nanoparticle; Plasmonic nanostructures; Plasmons; Probes; Prostate tissue; Protective shell; Proteins; Raman scattering; Raman signatures; Red-laser excitation; Reporter molecules; Selective detection; Self assembled monolayers; Self-assembled monolayer; SERS; Silver; Surface-enhanced Raman scattering; Target molecule; Tissue; Tumor diagnostics; Tumor suppressors, Agglomeration; Tumors, Optical properties
Erscheinungsdatum: 2010
Journal: Proceedings of SPIE - The International Society for Optical Engineering
Volumen: 7757
Nanoparticle probes for use in targeted detection schemes and readout by surface-enhanced Raman scattering (SERS) comprise a metal core, Raman reporter molecules and a protective shell. One design of SERS labels specifically optimized for biomedical applications in conjunction with red laser excitation is based on tunable gold/silver nanoshells, which are completely covered by a self-assembled monolayer (SAM) of Raman reporters. A shell around the SAMcoated metal core stabilizes the colloid and prevents particle aggregation. The optical properties and SERS efficiencies of these plasmonic nanostructures are characterized both experimentally and theoretically. Subsequent bioconjugation of SERS probes to ligands such as antibodies is a prerequisite for the selective detection of the corresponding target molecule via the characteristic Raman signature of the label. Biomedical imaging applications of SERS-labeled antibodies for tumor diagnostics by SERS microscopy are presented, using the localization of the tumor suppressor p63 in prostate tissue sections as an example. © 2010 SPIE.
Conference of Plasmonics: Metallic Nanostructures and Their Optical Properties VIII ; Conference Date: 1 August 2010 Through 5 August 2010; Conference Code:84167
ISBN: 9780819482532
ISSN: 0277786X
DOI: 10.1117/12.859253
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