Single channel analysis of membrane proteins in artificial bilayer membranes
| Autor(en): | Bartsch, P. Harsman, A. Wagner, R. |
Herausgeber: | Rapaport, D. Herrmann, J.M. |
Stichwörter: | artificial membrane; Bilayer; channel gating; electrode; Electrodes; Electrophysiology; Ion channel; laboratory device; lipid bilayer; Lipid Bilayers; membrane electrophysiology; Membrane Potentials; Membrane protein; membrane protein, article; Membrane Proteins; methodology; priority journal; procedures; protein analysis; protein function; Single channel analysis; single channel analysis, Electric Conductivity | Erscheinungsdatum: | 2013 | Journal: | Methods in Molecular Biology | Volumen: | 1033 | Startseite: | 345 | Seitenende: | 361 | Zusammenfassung: | The planar lipid bilayer technique is a powerful experimental approach for electrical single channel recordings of pore-forming membrane proteins in a chemically well-defined and easily modifiable environment. Here we provide a general survey of the basic materials and procedures required to set up a robust bilayer system and perform electrophysiological single channel recordings of reconstituted proteins suitable for the in-depth characterization of their functional properties. © 2013 Springer Science+Business Media, LLC. |
ISBN: | 9781627034869 | ISSN: | 10643745 | DOI: | 10.1007/978-1-62703-487-6_22 | Externe URL: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84884199986&doi=10.1007%2f978-1-62703-487-6_22&partnerID=40&md5=98ced9b468b1e7f03582062b442f8984 |
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geprüft am 01.06.2024
