Two-channel microfluidic CARS - Experimental quantification of pure vibrational contrast in CARS images
Autor(en): | Bergner, G. Henkel, T. Akimov, D. Dietzek, B. Schlücker, S. Bartelt, H. Popp, J. |
Stichwörter: | Acousto-optical programmable dispersive filters; Acoustooptic modulators; Analytes; Bio medical diagnostics; Broadband light sources; Coherent anti-Stokes Raman; Coherent anti-Stokes Raman microscopy; Coherent anti-Stokes Raman scattering; Coherent anti-Stokes Raman scattering microscopy; Concentration-dependent; Crystal whiskers; Deuterium; Deuterium compounds; Drug compounds; Drugs; Electronic switching; Experimental arrangement; Isotopomers; Light sources; Microfluidic chip; Microfluidics; Molecular concentration; Non-invasive; Nonlinear optics; Nonresonant; Photonic crystal fibers; Photonic crystals; Quantitative detection; Raman bands; Raman microspectroscopy; Raman resonances; Raman spectroscopy; Signal generation; Signal generators, Raman scattering; Tunable wavelength filters; Two-channel; Vibrational signals, Coherent scattering | Erscheinungsdatum: | 2011 | Journal: | Progress in Biomedical Optics and Imaging - Proceedings of SPIE | Volumen: | 8087 | Zusammenfassung: | The combination of linear and nonlinear Raman microspectroscopy has been established to be a powerful tool for biomedical diagnostics. In this contribution we discuss our recent approaches towards CARS (coherent anti-Stokes Raman scattering) based quantification of analytes, which is generally complicated by the CARS-signal strength dependence on the square of the molecular concentration and on the interplay between a molecular-specific vibrational signal and a nonresonant contribution in the signal generation. Due to these complications the quantification of analytes presents a major challenge in CARS microscopy. Here we discuss two recently developed approaches, i.e. on the one hand a simplified setup for coherent anti-Stokes Raman scattering (CARS) microscopy, which allows for recording CARS images with 30 cm-1 excitation bandwidth for probing Raman bands between 500 and 900 cm-1 with minimal requirements for alignment. This experimental arrangement is based on electronic switching between CARS images recorded at different Raman resonances by combining a photonic crystal fiber (PCF) as broad-band light source and an acoustooptical programmable dispersive filter (AOPDF) as tunable wavelength filter. On the other hand, we discuss how the introduction of carbon-deuterium (C-D) bonds into drug compounds constitutes a non-invasive labeling approach that allows for higher intrinsic CARS contrast to be obtained. The quantitative detection of C-deuterated drugs by Raman microspectroscopy and CARS microscopy is examined. Concentration-dependent studies on drugs with aliphatic and aromatic C-D moieties were performed in a two-channel microfluidic chip, using the corresponding non-deuterated (C-H) isotopomers as an internal reference. © 2011 SPIE-OSA. |
Beschreibung: | Conference of Clinical and Biomedical Spectroscopy and Imaging II ; Conference Date: 24 May 2011 Through 26 May 2011; Conference Code:85605 |
ISBN: | 9780819486844 | ISSN: | 16057422 | DOI: | 10.1117/12.889925 | Externe URL: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-79960506096&doi=10.1117%2f12.889925&partnerID=40&md5=08c199ea700ebb8647f9f96908e9a6fd |
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