Red-mediated recombineering of Salmonella enterica genomes
| Autor(en): | Czarniak, F. Hensel, M. |
Stichwörter: | DNA, 9007-49-2; DNA; Recombinases; DNA; recombinase, bacterial genome; Enterobacteria phage lambda; enzymology; genetic engineering; genetics; homologous recombination; metabolism; mutagenesis; procedures; Salmonella enterica, Bacteriophage lambda; DNA; Genetic Engineering; Genome, Bacterial; Homologous Recombination; Mutagenesis; Recombinases; Salmonella enterica | Erscheinungsdatum: | 2015 | Journal: | Methods in molecular biology (Clifton, N.J.) | Volumen: | 1225 | Startseite: | 63 | Seitenende: | 79 | Zusammenfassung: | The mutagenesis of enterobacterial genomes using phage λ Red recombinase functions is a rapid and versatile experimental tool. In addition to the rapid generation of deletions in the genome of Salmonella enterica, variations of the method allow site-directed mutagenesis, generation of reporter fusions, generation of chimeric genes, or transplantation of regulatory elements directly in the chromosome. We describe the application of these approaches with focus on practical aspects and critical steps. |
ISSN: | 19406029 | DOI: | 10.1007/978-1-4939-1625-2_4 | Externe URL: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84954623218&doi=10.1007%2f978-1-4939-1625-2_4&partnerID=40&md5=3e4009cd7165c78c9d8b276b1f8c69ee |
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