3D-localization microscopy and tracking of FoF1-ATP synthases in living bacteria
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Renz, A. | |
dc.contributor.author | Renz, M. | |
dc.contributor.author | Klütsch, D. | |
dc.contributor.author | Deckers-Hebestreit, G. | |
dc.contributor.author | Börsch, M. | |
dc.contributor.editor | Gregor, I. | |
dc.contributor.editor | Gryczynski, Z.K. | |
dc.contributor.editor | Koberling, F. | |
dc.contributor.editor | Enderlein, J. | |
dc.contributor.editor | Erdmann, R. | |
dc.date.accessioned | 2021-12-23T16:32:34Z | - |
dc.date.available | 2021-12-23T16:32:34Z | - |
dc.date.issued | 2015 | |
dc.identifier.isbn | 9781628414219 | |
dc.identifier.issn | 16057422 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/17413 | - |
dc.description | Conference of Single Molecule Spectroscopy and Superresolution Imaging VIII ; Conference Date: 7 February 2015 Through 8 February 2015; Conference Code:112031 | |
dc.description.abstract | FoF1-ATP synthases are membrane-embedded protein machines that catalyze the synthesis of adenosine triphosphate. Using photoactivation-based localization microscopy (PALM) in TIR-illumination as well as structured illumination microscopy (SIM), we explore the spatial distribution and track single FoF1-ATP synthases in living E. coli cells under physiological conditions at different temperatures. For quantitative diffusion analysis by mean-squared-displacement measurements, the limited size of the observation area in the membrane with its significant membrane curvature has to be considered. Therefore, we applied a 'sliding observation window' approach (M. Renz et al., Proc. SPIE 8225, 2012) and obtained the one-dimensional diffusion coefficient of FoF1-ATP synthase diffusing on the long axis in living E. coli cells. © 2015 SPIE. | |
dc.description.sponsorship | PicoQuant; The Society of Photo-Optical Instrumentation Engineers (SPIE) | |
dc.language.iso | en | |
dc.publisher | SPIE | |
dc.relation.ispartof | Progress in Biomedical Optics and Imaging - Proceedings of SPIE | |
dc.subject | Adenosinetriphosphate | |
dc.subject | Biosynthesis | |
dc.subject | Diffusion | |
dc.subject | Escherichia coli | |
dc.subject | FoF1-ATP synthase | |
dc.subject | Mean squared displacement | |
dc.subject | Molecules | |
dc.subject | Observation window | |
dc.subject | Optical resolving power, ATP synthase | |
dc.subject | PALM | |
dc.subject | Physiological condition | |
dc.subject | Single particle tracking | |
dc.subject | Structured illumination microscopies (SIM) | |
dc.subject | Super resolution imaging, Palmprint recognition | |
dc.subject | Superresolution imaging | |
dc.title | 3D-localization microscopy and tracking of FoF1-ATP synthases in living bacteria | |
dc.type | conference paper | |
dc.identifier.doi | 10.1117/12.2080981 | |
dc.identifier.scopus | 2-s2.0-84930463793 | |
dc.identifier.url | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84930463793&doi=10.1117%2f12.2080981&partnerID=40&md5=c7e01ee6a4c576f2c83516db05dae065 | |
dc.description.volume | 9331 | |
dcterms.isPartOf.abbreviation | Progr. Biomed. Opt. Imaging Proc. SPIE | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | DeGa700 | - |
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