Live cell imaging of intracellular Salmonella enterica
Autor(en): | Kehl, A. Hensel, M. |
Stichwörter: | Confocal microscopy; Intracellular pathogen; Salmonella-containing vacuole; Salmonella-induced filaments | Erscheinungsdatum: | 2014 | Herausgeber: | Springer New York | Journal: | Salmonella: Methods and Protocols: Second Edition | Startseite: | 199 | Seitenende: | 225 | Zusammenfassung: | During the intracellular phase of the pathogenic lifestyle, Salmonella enterica massively alters the endosomal system of its host cells. Two hallmarks are the remodeling of phagosomes into the Salmonella-containing vacuole (SCV) as a replicative niche, and the formation of tubular structures, such as Salmonella-induced filaments (SIFs). To study the dynamics and the fate of these Salmonella-specific compartments, live cell imaging (LCI) is a method of choice. In this chapter, we compare currently used microscopy techniques and focus on considerations and requirements specific for LCI. Detailed protocols for LCI of Salmonella infection with either confocal laser scanning microscopy (CLSM) or spinning disk confocal microscopy (SDCM) are provided. © Springer Science+Business Media New York 2015. All rights are reserved. |
ISBN: | 9781493916252 9781493916245 |
DOI: | 10.1007/978-1-4939-1625-2_13 | Externe URL: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84921786674&doi=10.1007%2f978-1-4939-1625-2_13&partnerID=40&md5=8d26649ef50aedc087736baf5b5275da |
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