Lack of malate valve capacities lead to improved n-assimilation and growth in transgenic A. Thaliana plants
| Autor(en): | Selinski, J. Scheibe, R. |
Stichwörter: | adenosine triphosphate, 15237-44-2, 56-65-5, 987-65-5; glutamate synthase (NADH), 65589-88-0; glyceraldehyde 3 phosphate dehydrogenase, 9001-50-7; malate dehydrogenase, 9001-64-3; malic acid, 149-61-1, 6915-15-7; nitrogen, 7727-37-9; oxidoreductase, 9035-73-8, 9035-82-9, 9037-80-3, 9055-15-6; Adenosine Triphosphate; Amino Acid Oxidoreductases; Arabidopsis Proteins; glutamate synthase (ferredoxin); Glutamate Synthase (NADH); Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating); Malate Dehydrogenase; Malates; malic acid; Nitrogen; Amino Acid Oxidoreductases; Ammonium assimilation; Arabidopsis; Arabidopsis protein; Arabidopsis Proteins; Arabidopsis thaliana, adenosine triphosphate; chloroplast; Chloroplasts; Energy Metabolism; Energy supply; glutamate synthase (ferredoxin); Glutamate Synthase (NADH); glyceraldehyde 3 phosphate dehydrogenase; Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating); Glycolysis; growth, development and aging; Malate Dehydrogenase; Malate valve; Malates; malic acid; malic acid derivative; metabolism; Nitrate assimilation; Nitrogen; oxidoreductase, Arabidopsis; Plants, Genetically Modified; Plastidial glycolysis; Redox-balance; transgenic plant, Adenosine Triphosphate | Erscheinungsdatum: | 2014 | Herausgeber: | Landes Bioscience | Journal: | Plant Signaling and Behavior | Volumen: | 9 | Ausgabe: | MAY | Zusammenfassung: | In this study we analyzed the relationship between malate valve capacities, n-assimilation, and energy metabolism. We used transgenic plants either lacking the chloroplast NADP-dependent malate dehydrogenase or mutants with a decreased transcript level of the plastid-localized NAD-dependent malate dehydrogenase. Plants were grown on nitrate or ammonium, respectively, as the sole N-source and transcripts were analyzed by qRT-PCR. We could show that the lack of malate valve capacities enhances N-assimilation and plastidial glycolysis by increasing transcript levels of Fd-GOGATs or NADH-GOGAT and plastidic NAD-GAPDHs (GapCps), respectively. Based on our results, we conclude that the lack of malate valve capacities is balanced by an increase of the activity of plastid-localized glycolysis in order to cover the high demand for plastidial ATP, stressing the importance of the plastids for energy metabolism in plant cells. © 2014 Landes Bioscience. |
ISSN: | 15592316 | DOI: | 10.4161/psb.29057 | Externe URL: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84903530928&doi=10.4161%2fpsb.29057&partnerID=40&md5=cb75fce73e79e4a81ae9fc11c511fc17 |
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