Characterization of truncated forms of the KdpD protein, the sensor kinase of the K+-translocating Kdp system of Escherichia coli

Autor(en): Puppe, W
Zimmann, P
Jung, K
Lucassen, M
Altendorf, K 
Stichwörter: ATPASE; AUTOPHOSPHORYLATION; Biochemistry & Molecular Biology; CONTROL EXPRESSION; IDENTIFICATION; OPERON EXPRESSION; OSMOREGULATION; PHOSPHORYLATION; POTASSIUM-TRANSPORT; REGULATOR; SIGNAL TRANSDUCTION
Erscheinungsdatum: 1996
Herausgeber: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Journal: JOURNAL OF BIOLOGICAL CHEMISTRY
Volumen: 271
Ausgabe: 40
Startseite: 25027
Seitenende: 25034
Zusammenfassung: 
The expression of the kdpFABC operon, coding for the K+-translocating Kdp system, is controlled by the two regulatory proteins, KdpD and KdpE, which belong to the group of sensor kinase/response regulator systems. This study describes the construction and analysis of KdpD sensor kinases, in which different deletions in the N-terminal part of the protein were introduced. Truncated KdpD proteins, in which the membrane-spanning segments were deleted, had lost their phosphorylation capacity. Truncated KdpD proteins, in which the four membrane-spanning helices were untouched, were still phosphorylated, and the phosphoryl group could be transferred to the response regulator KdpE in vitro. Furthermore, these truncated KdpD proteins cause dephosphorylation of KdpE(P), which is comparable with that of the wild-type protein. To investigate the effect of the deletions on signal transduction in vivo the corresponding kdp genes were transferred to the chromosome, Growth studies with the mutant strains are in accord with the data obtained from the in vitro studies. Furthermore, kdp expression was investigated using a KdpA-LacZ fusion. The data obtained support the notion that the extent of hdp expression is modulated by the N-terminal part of KdpD.
ISSN: 00219258
DOI: 10.1074/jbc.271.40.25027

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