7-DEAZA-2'-DEOXYADENOSINE AND 3-DEAZA-2'-DEOXYADENOSINE REPLACING DA WITHIN D(A6)-TRACTS - DIFFERENTIAL BENDING AT 3'-JUNCTIONS AND 5'-JUNCTIONS OF D(A6)BULLET-D(T6) AND B-DNA

DC FieldValueLanguage
dc.contributor.authorSEELA, F
dc.contributor.authorGREIN, T
dc.date.accessioned2021-12-23T15:56:31Z-
dc.date.available2021-12-23T15:56:31Z-
dc.date.issued1992
dc.identifier.issn03051048
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/2381-
dc.description.abstract7-Deaza-2'-deoxyadenosine (1, c7A(d)) and 3-deaza-2'-deoxyadenosine (2, c3A(d)) have been incorporated into d(AAAAAA) tracts replacing dA at various positions within oligonucleotides. For this purpose suitably protected phosphonates have been prepared and oligonucleotides were synthesized on solid-phase. The oligomers were hybridized with their cognate strands. The duplexes were phosphorylated at OH-5' by polynucleotide kinase and self-ligated to multimers employing T4 DNA ligase. Oligomerized DNA-fragments were analyzed by polyacrylamide gel electrophoresis and the bending was determined from anomalies of electrophoretic mobility. Replacement of dA by c3A(d) decreased the bending more than replacement by c7A(d). Reduction of bending was much stronger when the modified nucleosides replaced one or several dA residues at the 3'-site of an d(AAAAAA)-tract whereas replacement at the 5'-site showed no significant influence [1, 2].
dc.language.isoen
dc.publisherOXFORD UNIV PRESS UNITED KINGDOM
dc.relation.ispartofNUCLEIC ACIDS RESEARCH
dc.subject2'-DEOXYTUBERCIDIN
dc.subjectBASE
dc.subjectBiochemistry & Molecular Biology
dc.subjectCHROMATIN DNA
dc.subjectCURVED DNA
dc.subjectGLYCOSYLATION
dc.subjectHYDRATION
dc.subjectMINOR-GROOVE
dc.subjectOLIGONUCLEOTIDES
dc.subjectSEQUENCE
dc.subjectTHYMINE TRACTS
dc.title7-DEAZA-2'-DEOXYADENOSINE AND 3-DEAZA-2'-DEOXYADENOSINE REPLACING DA WITHIN D(A6)-TRACTS - DIFFERENTIAL BENDING AT 3'-JUNCTIONS AND 5'-JUNCTIONS OF D(A6)BULLET-D(T6) AND B-DNA
dc.typejournal article
dc.identifier.doi10.1093/nar/20.9.2297
dc.identifier.isiISI:A1992HV12700013
dc.description.volume20
dc.description.issue9
dc.description.startpage2297
dc.description.endpage2306
dc.publisher.placeWALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP
dcterms.isPartOf.abbreviationNucleic Acids Res.
dcterms.oaStatusGreen Published
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