Direct interaction of subunits a and b of the F-0 complex of Escherichia coli ATP synthase by forming an ab(2) subcomplex

DC FieldValueLanguage
dc.contributor.authorStalz, WD
dc.contributor.authorGreie, JC
dc.contributor.authorDeckers-Hebestreit, G
dc.contributor.authorAltendorf, K
dc.date.accessioned2021-12-23T15:56:43Z-
dc.date.available2021-12-23T15:56:43Z-
dc.date.issued2003
dc.identifier.issn00219258
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/2485-
dc.description.abstractThe addition of a His(6) tag to the N terminus of subunit a of the F-0 complex of the Escherichia coli ATP synthase allowed the purification of an ab(2) subcomplex after solubilization of membranes with n-dodecyl-beta-D-maltoside and subsequent nickel-nitrilotriacetic acid affinity chromatography. After co-reconstitution of the ab(2) subcomplex with purified subunit c, passive proton translocation rates as well as coupled ATPase activities after binding of F-1 were measured that were comparable with those of wild type F-0. The interaction between subunits a and b, which has been shown to be stoichiometric and functional, is not triggered by any cross-linking reagent and therefore reflects subunit interactions occurring within the F-0 complex in vivo.
dc.language.isoen
dc.publisherAMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
dc.relation.ispartofJOURNAL OF BIOLOGICAL CHEMISTRY
dc.subjectADENOSINE-TRIPHOSPHATASE
dc.subjectBiochemistry & Molecular Biology
dc.subjectC OLIGOMER
dc.subjectCROSS-LINKING
dc.subjectF1F0
dc.subjectMECHANISM
dc.subjectMEMBRANE MOIETY
dc.subjectORGANIZATION
dc.subjectPROTON-TRANSLOCATING ATPASE
dc.subjectRECONSTITUTION
dc.subjectROTARY MOTOR
dc.titleDirect interaction of subunits a and b of the F-0 complex of Escherichia coli ATP synthase by forming an ab(2) subcomplex
dc.typejournal article
dc.identifier.doi10.1074/jbc.M302027200
dc.identifier.isiISI:000184155700099
dc.description.volume278
dc.description.issue29
dc.description.startpage27068
dc.description.endpage27071
dc.publisher.place9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA
dcterms.isPartOf.abbreviationJ. Biol. Chem.
dcterms.oaStatushybrid
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidDeGa700-
crisitem.author.netidAlKa770-
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