Putative role of an SLC45 H+/sugar cotransporter in mammalian spermatozoa

DC FieldValueLanguage
dc.contributor.authorVitavska, Olga
dc.contributor.authorWieczorek, Helmut
dc.date.accessioned2021-12-23T15:56:53Z-
dc.date.available2021-12-23T15:56:53Z-
dc.date.issued2017
dc.identifier.issn00316768
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/2596-
dc.description.abstractIn the present study, we describe the detection and analysis of a novel type of sugar transporter in mammalian spermatozoa. This transporter belongs to the SLC45 family for which two features are remarkable and distinguish it from other known families of sugar transporters. Firstly, SLC45 transporters recognise not only the monosaccharides glucose or fructose but also the disaccharide sucrose as a substrate. Secondly, the uptake of sugars is coupled to a proton gradient. Uptake experiments using radioactively labelled sucrose indicated a functional transporter of the SLC45 family in bull spermatozoa. Real-time PCR as well as Western blots demonstrated the occurrence of the SLC45 member A4 in mouse testis and sperms. Furthermore, immunocytochemical analysis of mouse tissues revealed that the signal of SLC45A4 was mainly located in the principle piece of spermatozoa. We postulate that the SLC45A4 transporter plays an important role in nutrition of spermatozoa during their maturation in the epididymis. Moreover, we suggest that knowledge about the presence of the SLC45A4 may be useful also for the methodical improvement of cryopreservation of mammalian spermatozoa.
dc.description.sponsorshipDeutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [VI 673/2-1, WI 698/7-1]; This work was supported by the Deutsche Forschungsgemeinschaft (VI 673/2-1, WI 698/7-1).
dc.language.isoen
dc.publisherSPRINGER
dc.relation.ispartofPFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
dc.subjectDOG SPERMATOZOA
dc.subjectEPIDIDYMIS
dc.subjectEXPRESSION
dc.subjectFIBROUS SHEATH
dc.subjectMETABOLISM
dc.subjectPhysiology
dc.subjectProton-coupled sugar transporter
dc.subjectSACCHAROMYCES-CEREVISIAE
dc.subjectSLC45A4
dc.subjectSPERM
dc.subjectSpermatozoa maturation
dc.subjectSUCROSE
dc.subjectTRANSPORTER
dc.subjectUNDERWHITE GENE
dc.titlePutative role of an SLC45 H+/sugar cotransporter in mammalian spermatozoa
dc.typejournal article
dc.identifier.doi10.1007/s00424-017-2024-9
dc.identifier.isiISI:000412490400003
dc.description.volume469
dc.description.issue11
dc.description.startpage1433
dc.description.endpage1442
dc.identifier.eissn14322013
dc.publisher.place233 SPRING ST, NEW YORK, NY 10013 USA
dcterms.isPartOf.abbreviationPflugers Arch.
dcterms.oaStatushybrid, Green Published
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidViOl437-
crisitem.author.netidWiHe990-
Show simple item record

Page view(s)

5
Last Week
0
Last month
0
checked on Apr 15, 2024

Google ScholarTM

Check

Altmetric