The Conserved ADAMTS-like Protein Lonely heart Mediates Matrix Formation and Cardiac Tissue Integrity

DC FieldValueLanguage
dc.contributor.authorDrechsler, Maik
dc.contributor.authorSchmidt, Ariane C.
dc.contributor.authorMeyer, Heiko
dc.contributor.authorPaululat, Achim
dc.date.accessioned2021-12-23T15:57:10Z-
dc.date.available2021-12-23T15:57:10Z-
dc.date.issued2013
dc.identifier.issn15537404
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/2761-
dc.description.abstractHere we report on the identification and functional characterization of the ADAMTS-like homolog lonely heart (loh) in Drosophila melanogaster. Loh displays all hallmarks of ADAMTSL proteins including several thrombospondin type 1 repeats (TSR1), and acts in concert with the collagen Pericardin (Prc). Loss of either loh or prc causes progressive cardiac damage peaking in the abolishment of heart function. We show that both proteins are integral components of the cardiac ECM mediating cellular adhesion between the cardiac tube and the pericardial cells. Loss of ECM integrity leads to an altered myo-fibrillar organization in cardiac cells massively influencing heart beat pattern. We show evidence that Loh acts as a secreted receptor for Prc and works as a crucial determinant to allow the formation of a cell and tissue specific ECM, while it does not influence the accumulation of other matrix proteins like Nidogen or Perlecan. Our findings demonstrate that the function of ADAMTS-like proteins is conserved throughout evolution and reveal a previously unknown interaction of these proteins with collagens.
dc.description.sponsorshipDFGGerman Research Foundation (DFG)European Commission [SFB944/TP7]; DAAD/IPID program; This study was funded by the DFG (SFB944/TP7) and the DAAD/IPID program. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.language.isoen
dc.publisherPUBLIC LIBRARY SCIENCE
dc.relation.ispartofPLOS GENETICS
dc.subjectCELL-ADHESION
dc.subjectDROSOPHILA-MELANOGASTER
dc.subjectECTOPIA LENTIS
dc.subjectEXTRACELLULAR-MATRIX
dc.subjectGenetics & Heredity
dc.subjectIV
dc.subjectLAMININ
dc.subjectMETALLOPROTEINASES
dc.subjectMORPHOGENESIS
dc.subjectMUTATIONS
dc.subjectSEQUENCE
dc.titleThe Conserved ADAMTS-like Protein Lonely heart Mediates Matrix Formation and Cardiac Tissue Integrity
dc.typejournal article
dc.identifier.doi10.1371/journal.pgen.1003616
dc.identifier.isiISI:000322321100021
dc.description.volume9
dc.description.issue7
dc.contributor.orcid0000-0002-3304-4523
dc.contributor.orcid0000-0002-8845-6859
dc.publisher.place1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
dcterms.isPartOf.abbreviationPLoS Genet.
dcterms.oaStatusGreen Published, Green Submitted, gold
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0002-8845-6859-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidPaAc947-
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