Elucidating the biosynthesis of chitin filaments and their configuration with specific proteins and electron microscopy

DC FieldValueLanguage
dc.contributor.authorSiemieniewicz, Krzysztof W.
dc.contributor.authorKajla, Mayur K.
dc.contributor.authorSchrempf, Hildgund
dc.date.accessioned2021-12-23T15:57:12Z-
dc.date.available2021-12-23T15:57:12Z-
dc.date.issued2007
dc.identifier.issn16165187
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/2786-
dc.description.abstractTo deepen the knowledge of chitin synthesis, a yeast mutant has been used as a model. Purified chitin synthase I-containing vesicles (chitosomes) with a diameter of 85 to 120 nm are identified by electron microscopy to eject tiny fibers upon addition of UDP-N-acetylglucosamine. The filigree of extruded filaments fused gradually into a large three-dimensional network, which is degradable by a chitinase. The network is targeted and restructured by the Streptomyces chitin-binding protein CHB1, which has a very high affinity only for alpha-chitin. Within the chitosomes, filaments are found to be highly condensed within consecutive oval fibroids, which are specifically targeted by the alpha-chitin-binding protein. The presented data give new insights to the generation of chitin filaments with an antiparallel (alpha) configuration.
dc.language.isoen
dc.publisherWILEY-V C H VERLAG GMBH
dc.relation.ispartofMACROMOLECULAR BIOSCIENCE
dc.subjectALPHA-CHITIN
dc.subjectBINDING
dc.subjectBiochemistry & Molecular Biology
dc.subjectCHB1
dc.subjectCHITOSOMES
dc.subjectFUNGI
dc.subjectGENE
dc.subjectGROWTH
dc.subjectLOCALIZATION
dc.subjectMaterials Science
dc.subjectMaterials Science, Biomaterials
dc.subjectPolymer Science
dc.subjectSYNTHETASE
dc.titleElucidating the biosynthesis of chitin filaments and their configuration with specific proteins and electron microscopy
dc.typejournal article
dc.identifier.doi10.1002/mabi.200600180
dc.identifier.isiISI:000244044700004
dc.description.volume7
dc.description.issue1
dc.description.startpage40
dc.description.endpage47
dc.identifier.eissn16165195
dc.publisher.placePOSTFACH 101161, 69451 WEINHEIM, GERMANY
dcterms.isPartOf.abbreviationMacromol. Biosci.
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidScHi752-
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