SUGGESTIONS FOR AN INDEX OF ENCHYTRAEID SPECIES (OLIGOCHAETA) BASED ON GENERAL PROTEIN-PATTERNS

Autor(en): WESTHEIDE, W
BROCKMEYER, V
Stichwörter: ENCHYTRAEIDAE; GEL-ELECTROPHORESIS; IDENTIFICATION; ISOELECTRIC FOCUSING (IEF); PROTEIN PATTERN INDEX; SPECIES DIFFERENTIATION; Zoology
Erscheinungsdatum: 1992
Herausgeber: BLACKWELL WISSENSCHAFTS-VERLAG GMBH
Journal: ZEITSCHRIFT FUR ZOOLOGISCHE SYSTEMATIK UND EVOLUTIONSFORSCHUNG
Volumen: 30
Ausgabe: 2
Startseite: 89
Seitenende: 99
Zusammenfassung: 
Polyacrylamide gel isoelectric focusing (PAGIF) of general proteins was successfully used as a taxonomic method in the oligochaete genus Enchytraeus. Single specimens of 22 different populations were investigated, originating from various areas and belonging to an unknown number of species. Animals of monomorphic populations showed identical band patterns. Variability of band patterns within polymorphic populations was relatively low and did not limit applicability of the method for species separation. Patterns were constant and independent of origin, culture conditions and age. They were sufficiently reproducible if experimental conditions were carefully kept constant. Patterns obtained from specimens fixed in 90 % ethanol were identical with those of living or deep-frozen material. It is suggested to establish a comprehensive index based on protein patterns for the genus Enchytraeus and other enchytraeid taxa difficult to distinguish on morphological features. The index should contain protein data of the entire spectrum of species together with morphological and other available data, listed in a catalogue and deposited like a type collection.
ISSN: 00443808

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