Role of Host Cell-Derived Amino Acids in Nutrition of Intracellular Salmonella enterica

DC FieldValueLanguage
dc.contributor.authorPopp, Jasmin
dc.contributor.authorNoster, Janina
dc.contributor.authorBusch, Kim
dc.contributor.authorKehl, Alexander
dc.contributor.authorzur Hellen, Gero
dc.contributor.authorHensel, Michael
dc.date.accessioned2021-12-23T15:57:24Z-
dc.date.available2021-12-23T15:57:24Z-
dc.date.issued2015
dc.identifier.issn00199567
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/2904-
dc.description.abstractThe facultative intracellular pathogen Salmonella enterica resides in a specific membrane-bound compartment termed the Salmonella- containing vacuole (SCV). Despite being segregated from access to metabolites in the host cell cytosol, Salmonella is able to efficiently proliferate within the SCV. We set out to unravel the nutritional supply of Salmonella in the SCV with focus on amino acids. We studied the availability of amino acids by the generation of auxotrophic strains for alanine, asparagine, aspartate, glutamine, and proline in a macrophage cell line (RAW264.7) and an epithelial cell line (HeLa) and examined access to extracellular nutrients for nutrition. Auxotrophies for alanine, asparagine, or proline attenuated intracellular replication in HeLa cells, while aspartate, asparagine, or proline auxotrophies attenuated intracellular replication in RAW264.7 macrophages. The different patterns of intracellular attenuation of alanine-or aspartate-auxotrophic strains support distinct nutritional conditions in HeLa cells and RAW264.7 macrophages. Supplementation of medium with individual amino acids restored the intracellular replication of mutant strains auxotrophic for asparagine, proline, or glutamine. Similarly, a mutant strain deficient in succinate dehydrogenase was complemented by the extracellular addition of succinate. Complementation of the intracellular replication of auxotrophic Salmonella by external amino acids was possible if bacteria were proficient in the induction of Salmonella- induced filaments (SIFs) but failed in a SIF-deficient background. We propose that the ability of intracellular Salmonella to redirect host cell vesicular transport provides access of amino acids to auxotrophic strains and, more generally, is essential to continuously supply bacteria within the SCV with nutrients.
dc.description.sponsorshipDFG priority program 1316, Host-Adapted Metabolism of Bacteria PathogensGerman Research Foundation (DFG) [HE1964/14-2]; This work was supported by grant HE1964/14-2 as part of the DFG priority program 1316, Host-Adapted Metabolism of Bacteria Pathogens.
dc.language.isoen
dc.publisherAMER SOC MICROBIOLOGY
dc.relation.ispartofINFECTION AND IMMUNITY
dc.subjectCULTURE
dc.subjectESCHERICHIA-COLI
dc.subjectGENES
dc.subjectGLUTAMINE
dc.subjectGROWTH
dc.subjectImmunology
dc.subjectInfectious Diseases
dc.subjectINHIBITION
dc.subjectPATHOGENICITY
dc.subjectPROLINE
dc.subjectSYSTEM
dc.subjectTYPHIMURIUM
dc.titleRole of Host Cell-Derived Amino Acids in Nutrition of Intracellular Salmonella enterica
dc.typejournal article
dc.identifier.doi10.1128/IAI.00624-15
dc.identifier.isiISI:000364944400002
dc.description.volume83
dc.description.issue12
dc.description.startpage4466
dc.description.endpage4475
dc.contributor.orcid0000-0001-6604-6253
dc.contributor.orcid0000-0002-1127-8847
dc.contributor.orcid0000-0002-8802-6605
dc.identifier.eissn10985522
dc.publisher.place1752 N ST NW, WASHINGTON, DC 20036-2904 USA
dcterms.isPartOf.abbreviationInfect. Immun.
dcterms.oaStatusGreen Published, Bronze
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0001-6604-6253-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidHeMi480-
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