Proton-associated sucrose transport of mammalian solute carrier family 45: an analysis in Saccharomyces cerevisiae

Autor(en): Bartoelke, Rabea
Heinisch, Juergen J.
Wieczorek, Helmut 
Vitavska, Olga 
Stichwörter: Biochemistry & Molecular Biology; D-MANNOSE TRANSPORT; EXPRESSION; INTESTINAL GLUCOSE-ABSORPTION; melanin synthesis; MUCOSAL SURFACE PH; MUTATIONS; OCULOCUTANEOUS ALBINISM; RAT SMALL-INTESTINE; SLC45A2; SLC45A3; SLC45A4; solute carrier family 45; sucrose transport; UNDERWHITE GENE; YEAST
Erscheinungsdatum: 2014
Herausgeber: PORTLAND PRESS LTD
Journal: BIOCHEMICAL JOURNAL
Volumen: 464
Ausgabe: 2
Startseite: 193
Seitenende: 201
Zusammenfassung: 
The members of the solute carrier 45 (SLC45) family have been implicated in the regulation of glucose homoeostasis in the brain (SLC45A1), with skin and hair pigmentation (SLC45A2), and with prostate cancer and myelination (SLC45A3). However, apart from SLC45A1, a proton-associated glucose transporter, the function of these proteins is still largely unknown, although sequence similarities to plant sucrose transporters mark them as a putative sucrose transporter family. Heterologous expression of the three members SLC45A2, SLC45A3 and SLC45A4 in Saccharomyces cerevisiae confirmed that they are indeed sucrose transporters. [C-14]Sucrose-uptake measurements revealed intermediate transport affinities with K-m values of approximately 5 mM, Transport activities were best under slightly acidic conditions and were inhibited by the protonophore carbonyl cyanide m-chlorophenylhydrazonc, demonstrating an H+ -coupled transport mechanism. Na+, on the other hand, had no effect on sucrose transport. Competitive inhibition assays indicated a possible transport also of glucose and fructose. Real-time PCR of mouse tissues confirmed mRNA expression of SLC45A2 in eyes and skin and of SLC45A3 primarily in the prostate, but also in other tissues, whereas SLC45A4 showed a predominantly ubiquitous expression. Altogether the results provide new insights into the physiological significance of SLC45 family members and challenge existing concepts of mammalian sugar transport, as they (i) transport a disaccharide, and (ii) perform secondary active transport in a proton-dependent manner.
ISSN: 02646021
DOI: 10.1042/BJ20140572

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