Proton-associated sucrose transport of mammalian solute carrier family 45: an analysis in Saccharomyces cerevisiae

DC FieldValueLanguage
dc.contributor.authorBartoelke, Rabea
dc.contributor.authorHeinisch, Juergen J.
dc.contributor.authorWieczorek, Helmut
dc.contributor.authorVitavska, Olga
dc.date.accessioned2021-12-23T15:57:24Z-
dc.date.available2021-12-23T15:57:24Z-
dc.date.issued2014
dc.identifier.issn02646021
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/2908-
dc.description.abstractThe members of the solute carrier 45 (SLC45) family have been implicated in the regulation of glucose homoeostasis in the brain (SLC45A1), with skin and hair pigmentation (SLC45A2), and with prostate cancer and myelination (SLC45A3). However, apart from SLC45A1, a proton-associated glucose transporter, the function of these proteins is still largely unknown, although sequence similarities to plant sucrose transporters mark them as a putative sucrose transporter family. Heterologous expression of the three members SLC45A2, SLC45A3 and SLC45A4 in Saccharomyces cerevisiae confirmed that they are indeed sucrose transporters. [C-14]Sucrose-uptake measurements revealed intermediate transport affinities with K-m values of approximately 5 mM, Transport activities were best under slightly acidic conditions and were inhibited by the protonophore carbonyl cyanide m-chlorophenylhydrazonc, demonstrating an H+ -coupled transport mechanism. Na+, on the other hand, had no effect on sucrose transport. Competitive inhibition assays indicated a possible transport also of glucose and fructose. Real-time PCR of mouse tissues confirmed mRNA expression of SLC45A2 in eyes and skin and of SLC45A3 primarily in the prostate, but also in other tissues, whereas SLC45A4 showed a predominantly ubiquitous expression. Altogether the results provide new insights into the physiological significance of SLC45 family members and challenge existing concepts of mammalian sugar transport, as they (i) transport a disaccharide, and (ii) perform secondary active transport in a proton-dependent manner.
dc.description.sponsorshipDeutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [VI 673/2-1, WI 698/7-1]; Lichtenberg Ph.D. programme; This work was supported by the Deutsche Forschungsgemeinschaft [grant numbers VI 673/2-1, WI 698/7-1] and by the Lichtenberg Ph.D. programme.
dc.language.isoen
dc.publisherPORTLAND PRESS LTD
dc.relation.ispartofBIOCHEMICAL JOURNAL
dc.subjectBiochemistry & Molecular Biology
dc.subjectD-MANNOSE TRANSPORT
dc.subjectEXPRESSION
dc.subjectINTESTINAL GLUCOSE-ABSORPTION
dc.subjectmelanin synthesis
dc.subjectMUCOSAL SURFACE PH
dc.subjectMUTATIONS
dc.subjectOCULOCUTANEOUS ALBINISM
dc.subjectRAT SMALL-INTESTINE
dc.subjectSLC45A2
dc.subjectSLC45A3
dc.subjectSLC45A4
dc.subjectsolute carrier family 45
dc.subjectsucrose transport
dc.subjectUNDERWHITE GENE
dc.subjectYEAST
dc.titleProton-associated sucrose transport of mammalian solute carrier family 45: an analysis in Saccharomyces cerevisiae
dc.typejournal article
dc.identifier.doi10.1042/BJ20140572
dc.identifier.isiISI:000345265000003
dc.description.volume464
dc.description.issue2
dc.description.startpage193
dc.description.endpage201
dc.contributor.orcid0000-0003-2531-747X
dc.contributor.researcheridG-3801-2017
dc.contributor.researcheridO-3995-2016
dc.identifier.eissn14708728
dc.publisher.place1ST FLR, 10 QUEEN STREET PLACE, LONDON, ENGLAND
dcterms.isPartOf.abbreviationBiochem. J.
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidWiHe990-
crisitem.author.netidViOl437-
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