Rapid Transfer of Transmembrane Proteins for Single Molecule Dimerization Assays in Polymer-Supported Membranes

DC FieldValueLanguage
dc.contributor.authorRoder, Friedrich
dc.contributor.authorWilmes, Stephan
dc.contributor.authorRichter, Christian P.
dc.contributor.authorPiehler, Jacob
dc.date.accessioned2021-12-23T15:57:25Z-
dc.date.available2021-12-23T15:57:25Z-
dc.date.issued2014
dc.identifier.issn15548929
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/2910-
dc.description.abstractDimerization of transmembrane receptors is a key regulatory factor in cellular communication, which has remained challenging to study under well-defined conditions in vitro. We developed a novel strategy to explore membrane protein interactions in a controlled lipid environment requiring minute sample quantities. By rapid transfer of transmembrane proteins from mammalian cells into polymer-supported membranes, membrane proteins could be efficiently fluorescence labeled and reconstituted with very low background. Thus, differential ligand-induced dimerization of the type I interferon (IFN) receptor subunits IFNAR1 and IFNAR2 could be probed quantitatively at physiologically relevant concentrations by single molecule imaging. These measurements clearly support a regulatory role of the affinity of IFNs toward IFNAR1 for controlling the level of receptor dimerization.
dc.description.sponsorshipDeutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [PI 405/5, SFB 944]; European CommunityEuropean Commission [223608]; This project was supported by the Deutsche Forschungsgemeinschaft (PI 405/5 and SFB 944) and by the European Community's Seventh Framework Programme (FP7/2007-2013) under grant agreement No. 223608 (IFNaction).
dc.language.isoen
dc.publisherAMER CHEMICAL SOC
dc.relation.ispartofACS CHEMICAL BIOLOGY
dc.subjectACTIVATION
dc.subjectARCHITECTURE
dc.subjectBiochemistry & Molecular Biology
dc.subjectDIFFUSION
dc.subjectI INTERFERON RECEPTOR
dc.subjectLIPIDS
dc.subjectORGANIZATION
dc.subjectPLASMA-MEMBRANE
dc.subjectRECOGNITION
dc.subjectRECONSTITUTION
dc.subjectREVEALS
dc.titleRapid Transfer of Transmembrane Proteins for Single Molecule Dimerization Assays in Polymer-Supported Membranes
dc.typejournal article
dc.identifier.doi10.1021/cb5005806
dc.identifier.isiISI:000345551300005
dc.description.volume9
dc.description.issue11
dc.description.startpage2479
dc.description.endpage2484
dc.contributor.orcid0000-0002-4112-710X
dc.identifier.eissn15548937
dc.publisher.place1155 16TH ST, NW, WASHINGTON, DC 20036 USA
dcterms.isPartOf.abbreviationACS Chem. Biol.
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0002-2143-2270-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidPiJa938-
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