Rapid Transfer of Transmembrane Proteins for Single Molecule Dimerization Assays in Polymer-Supported Membranes
DC Element | Wert | Sprache |
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dc.contributor.author | Roder, Friedrich | |
dc.contributor.author | Wilmes, Stephan | |
dc.contributor.author | Richter, Christian P. | |
dc.contributor.author | Piehler, Jacob | |
dc.date.accessioned | 2021-12-23T15:57:25Z | - |
dc.date.available | 2021-12-23T15:57:25Z | - |
dc.date.issued | 2014 | |
dc.identifier.issn | 15548929 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/2910 | - |
dc.description.abstract | Dimerization of transmembrane receptors is a key regulatory factor in cellular communication, which has remained challenging to study under well-defined conditions in vitro. We developed a novel strategy to explore membrane protein interactions in a controlled lipid environment requiring minute sample quantities. By rapid transfer of transmembrane proteins from mammalian cells into polymer-supported membranes, membrane proteins could be efficiently fluorescence labeled and reconstituted with very low background. Thus, differential ligand-induced dimerization of the type I interferon (IFN) receptor subunits IFNAR1 and IFNAR2 could be probed quantitatively at physiologically relevant concentrations by single molecule imaging. These measurements clearly support a regulatory role of the affinity of IFNs toward IFNAR1 for controlling the level of receptor dimerization. | |
dc.description.sponsorship | Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [PI 405/5, SFB 944]; European CommunityEuropean Commission [223608]; This project was supported by the Deutsche Forschungsgemeinschaft (PI 405/5 and SFB 944) and by the European Community's Seventh Framework Programme (FP7/2007-2013) under grant agreement No. 223608 (IFNaction). | |
dc.language.iso | en | |
dc.publisher | AMER CHEMICAL SOC | |
dc.relation.ispartof | ACS CHEMICAL BIOLOGY | |
dc.subject | ACTIVATION | |
dc.subject | ARCHITECTURE | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | DIFFUSION | |
dc.subject | I INTERFERON RECEPTOR | |
dc.subject | LIPIDS | |
dc.subject | ORGANIZATION | |
dc.subject | PLASMA-MEMBRANE | |
dc.subject | RECOGNITION | |
dc.subject | RECONSTITUTION | |
dc.subject | REVEALS | |
dc.title | Rapid Transfer of Transmembrane Proteins for Single Molecule Dimerization Assays in Polymer-Supported Membranes | |
dc.type | journal article | |
dc.identifier.doi | 10.1021/cb5005806 | |
dc.identifier.isi | ISI:000345551300005 | |
dc.description.volume | 9 | |
dc.description.issue | 11 | |
dc.description.startpage | 2479 | |
dc.description.endpage | 2484 | |
dc.contributor.orcid | 0000-0002-4112-710X | |
dc.identifier.eissn | 15548937 | |
dc.publisher.place | 1155 16TH ST, NW, WASHINGTON, DC 20036 USA | |
dcterms.isPartOf.abbreviation | ACS Chem. Biol. | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-2143-2270 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | PiJa938 | - |
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geprüft am 15.05.2024