RAPID PROTON-TRANSFER UNDER FLASHING LIGHT AT BOTH FUNCTIONAL SIDES OF DARK-ADAPTED PHOTOSYSTEM-II PARTICLES
Autor(en): | BOGERSHAUSEN, O JUNGE, W |
Stichwörter: | Biochemistry & Molecular Biology; Biophysics; CENTER CORE PREPARATION; CORE PARTICLE; DARK ADAPTATION, REPETITIVE; ELECTRON TRANSFER; ELECTRON-PARAMAGNETIC-RES; EVOLVING APPARATUS; KINETICS; OXYGEN EVOLUTION; OXYGEN EVOLUTION CAPACITY; PH-DEPENDENCE; PHOTOSYNTHESIS; PHOTOSYNTHETIC WATER OXIDATION; PHOTOSYSTEM II; PROTON TRANSFER; RELEASE; SPINACH; THYLAKOID MEMBRANES; WATER OXIDATION | Erscheinungsdatum: | 1995 | Herausgeber: | ELSEVIER SCIENCE BV | Journal: | BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | Volumen: | 1230 | Ausgabe: | 3 | Startseite: | 177 | Seitenende: | 185 | Zusammenfassung: | By exposing dark-adapted Photosystem II core particles to a series of light flashes, we aimed at kinetic resolution of proton release during the four steps of water oxidation. The signal-to-noise ratio was improved by averaging under repetitive dark adaptation. The previously observed kinetic damping of pH-transients by particle aggregation was prevented by detergent. The complicating superimposition of protolytic events at the donor side (water oxidation) and at the acceptor side (quinone oxide-reduction) was unravelled by characterizing the rate constants of electron and proton transfer at the acceptor side (Q(A)(-) . nH(+) DCBQ --> Q(A) DCBQ(-) nH(+): k = 1.7 . 10(6) M(-1) s(-1)//2 DCBQ(-) 2H(+) --> DCBQ DCBQH(2): k = 4 . 10(8) M(-1) s(-1)). Contrasting with the pronounced period of four oscillations of the oxygen-evolving centre, the extent of proton release was practically constant. The apparent half-rise time of the stepped acidification was shortened upon lowering of the pH (250 mu s at pH 7.5, 70 mu s at pH 6.0 and 12 mu s at pH 5.2). This kinetic behaviour was independent of the nature and the concentration of the added pH-indicator. We conclude that this reflects the protolysis of several electrostatically interacting acids at the surface of the protein in response to a new positive charge on Y-Z(+), and persisting upon electron transfer from the manganese cluster to Y-Z(+). |
ISSN: | 00052728 | DOI: | 10.1016/0005-2728(95)00057-P |
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geprüft am 18.05.2024