Muscle Function Assessment Using a Drosophila Larvae Crawling Assay

Autor(en): Post, Yanina
Paululat, Achim 
Stichwörter: Biology; Crawling; Drosophila melanogaster; Life Sciences & Biomedicine - Other Topics; Locomotion; Muscle contraction; Video analysis
Erscheinungsdatum: 2018
Herausgeber: BIO-PROTOCOL
Journal: BIO-PROTOCOL
Volumen: 8
Ausgabe: 14
Zusammenfassung: 
Here we describe a simple method to measure larval muscle contraction and locomotion behavior. The method enables the user to acquire data, without the necessity of using expensive equipment (Rotstein et al., 2018). To measure contraction and locomotion behaviour, single larvae are positioned at the center of a humidified Petri dish. Larval movement is recorded over time using the movie function of a consumer digital camera. Subsequently, videos are analyzed using ImageJ (Rueden et al., 2017) for distance measurements and counting of contractions. Data are represented as box or scatter plots using GraphPad Prism ((c) GraphPad Software).
DOI: 10.21769/BioProtoc.2933

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