Identification of an animal sucrose transporter
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Meyer, Heiko | |
dc.contributor.author | Vitavska, Olga | |
dc.contributor.author | Wieczorek, Helmut | |
dc.date.accessioned | 2021-12-23T15:57:41Z | - |
dc.date.available | 2021-12-23T15:57:41Z | - |
dc.date.issued | 2011 | |
dc.identifier.issn | 00219533 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/3065 | - |
dc.description.abstract | According to a classic tenet, sugar transport across animal membranes is restricted to monosaccharides. Here, we present the first report of an animal sucrose transporter, SCRT, which we detected in Drosophila melanogaster at each developmental stage. We localized the protein in apical membranes of the late embryonic hindgut as well as in vesicular membranes of ovarian follicle cells. The fact that knockdown of SCRT expression results in significantly increased lethality demonstrates an essential function for the protein. Experiments with Saccharomyces cerevisiae as a heterologous expression system revealed that sucrose is a transported substrate. Because the knockout of SLC45A2, a highly similar protein belonging to the mammalian solute carrier family 45 (SLC45) causes oculocutaneous albinism and because the vesicular structures in which SCRT is located appear to contain melanin, we propose that these organelles are melanosome-like structures and that the transporter is necessary for balancing the osmotic equilibrium during the polymerization process of melanin by the import of a compatible osmolyte. In the hindgut epithelial cells, sucrose might also serve as a compatible osmolyte, but we cannot exclude the possibility that transport of this disaccharide also serves nutritional adequacy. | |
dc.description.sponsorship | DFG (Research training group)German Research Foundation (DFG) [612]; Hans Muhlenhoff foundation; This work was supported in part by the DFG (Research training group 612) and the Hans Muhlenhoff foundation. We thank Klaus W. Beyenbach (Cornell University) for valuable comments on the manuscript and Torsten Struck for giving support regarding the phylogenetic analysis. | |
dc.language.iso | en | |
dc.publisher | COMPANY BIOLOGISTS LTD | |
dc.relation.ispartof | JOURNAL OF CELL SCIENCE | |
dc.subject | Cell Biology | |
dc.subject | COMPATIBLE OSMOLYTE | |
dc.subject | Drosophila melanogaster | |
dc.subject | EXPRESSION | |
dc.subject | GENE FUSIONS | |
dc.subject | H+-ATPASE | |
dc.subject | INTERNAL DYNAMICS | |
dc.subject | PROTEIN | |
dc.subject | PROTON PUMP | |
dc.subject | Sucrose transporter | |
dc.subject | Sugar transport | |
dc.subject | SUGAR-TRANSPORT | |
dc.subject | TREHALOSE | |
dc.subject | YEAST | |
dc.title | Identification of an animal sucrose transporter | |
dc.type | journal article | |
dc.identifier.doi | 10.1242/jcs.082024 | |
dc.identifier.isi | ISI:000291048000004 | |
dc.description.volume | 124 | |
dc.description.issue | 12 | |
dc.description.startpage | 1984 | |
dc.description.endpage | 1991 | |
dc.contributor.orcid | 0000-0002-3304-4523 | |
dc.identifier.eissn | 14779137 | |
dc.publisher.place | BIDDER BUILDING, STATION RD, HISTON, CAMBRIDGE CB24 9LF, ENGLAND | |
dcterms.isPartOf.abbreviation | J. Cell Sci. | |
dcterms.oaStatus | Green Published, Bronze | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | ViOl437 | - |
crisitem.author.netid | WiHe990 | - |
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