IDENTIFICATION OF THE PUTATIVE HEXOSE-PHOSPHATE TRANSLOCATOR OF AMYLOPLASTS FROM CAULIFLOWER BUDS
| Autor(en): | BATZ, O SCHEIBE, R NEUHAUS, HE |
Stichwörter: | 3-PHOSPHOGLYCERATE; Biochemistry & Molecular Biology; ENVELOPE MEMBRANES; GLUCOSE-6-PHOSPHATE; INHIBITION; INORGANIC-PHOSPHATE; MESOPHYLL CHLOROPLASTS; PLASTIDS; PROTEINS; STARCH SYNTHESIS | Erscheinungsdatum: | 1993 | Herausgeber: | PORTLAND PRESS LTD | Journal: | BIOCHEMICAL JOURNAL | Volumen: | 294 | Ausgabe: | 1 | Startseite: | 15 | Seitenende: | 17 | Zusammenfassung: | Starch synthesis in amyloplasts isolated from cauliflower buds is strongly inhibited by the addition of micromolar concentrations of 4,4'-di-isothiocyano-2,2'-stilbenedisulphonic acid (DIDS). Using [H-3]DIDS it was possible to label specifically a 31.6 kDa membrane protein of the envelope fraction of isolated amyloplasts. The intensity of the radioactive label was decreased in the presence of glucose 6-phosphate or dihydroxyacetone phosphate, indicating that this protein might be the amyloplastic hexose-phosphate translocator. |
ISSN: | 02646021 | DOI: | 10.1042/bj2940015 |
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