DC Field | Value | Language |
dc.contributor.author | You, Changjiang | |
dc.contributor.author | Piehler, Jacob | |
dc.date.accessioned | 2021-12-23T15:57:54Z | - |
dc.date.available | 2021-12-23T15:57:54Z | - |
dc.date.issued | 2016 | |
dc.identifier.issn | 17460441 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/3197 | - |
dc.description.abstract | Introduction: The past decade has witnessed tremendous progress in surface micropatterning techniques for generating arrays of various types of biomolecules. Multiplexed protein micropatterning has tremendous potential for drug discovery providing versatile means for high throughput assays required for target and lead identification as well as diagnostics and functional screening for personalized medicine. However, ensuring the functional integrity of proteins on surfaces has remained challenging, in particular in the case of membrane proteins, the most important class of drug targets. Yet, generic strategies to control functional organization of proteins into micropatterns are emerging.Areas covered: This review includes an overview introducing the most common approaches for surface modification and functional protein immobilization. The authors present the key photo and soft lithography techniques with respect to compatibility with functional protein micropatterning and multiplexing capabilities. In the second part, the authors present the key applications of protein micropatterning techniques in drug discovery with a focus on membrane protein interactions and cellular signaling.Expert opinion: With the growing importance of target discovery as well as protein-based therapeutics and personalized medicine, the application of protein arrays can play a fundamental role in drug discovery. Yet, important technical breakthroughs are still required for broad application of these approaches, which will include invitro copying of proteins from cDNA arrays into micropatterns, direct protein capturing from single cells as well as protein microarrays in living cells. | |
dc.description.sponsorship | Deutsche Forschungsgemeinschaft (DFG)German Research Foundation (DFG); Part of this work was funded by the Deutsche Forschungsgemeinschaft (DFG). J Piehler is the co-inventor of tris-NTA patent EP1778651 entitled ``Multivalent chelators for modifying and organizing of target molecules'' (WO2005EP08124/DE20041038134/US20040614950P). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. | |
dc.language.iso | en | |
dc.publisher | TAYLOR & FRANCIS LTD | |
dc.relation.ispartof | EXPERT OPINION ON DRUG DISCOVERY | |
dc.subject | ARRAYS | |
dc.subject | CELL | |
dc.subject | cell patterning | |
dc.subject | COVALENT IMMOBILIZATION | |
dc.subject | DIP-PEN NANOLITHOGRAPHY | |
dc.subject | LITHOGRAPHY | |
dc.subject | membrane protein | |
dc.subject | MICROARRAYS | |
dc.subject | NEXT-GENERATION | |
dc.subject | Pharmacology & Pharmacy | |
dc.subject | protein array | |
dc.subject | protein immobilization | |
dc.subject | protein interaction | |
dc.subject | RECEPTOR TYROSINE KINASES | |
dc.subject | SELF-ASSEMBLED MONOLAYERS | |
dc.subject | SUPPORTED MEMBRANES | |
dc.subject | surface micropatterning | |
dc.title | Functional protein micropatterning for drug design and discovery | |
dc.type | review | |
dc.identifier.doi | 10.1517/17460441.2016.1109625 | |
dc.identifier.isi | ISI:000368024700001 | |
dc.description.volume | 11 | |
dc.description.issue | 1 | |
dc.description.startpage | 105 | |
dc.description.endpage | 119 | |
dc.contributor.orcid | 0000-0002-7839-6397 | |
dc.contributor.researcherid | L-3901-2014 | |
dc.identifier.eissn | 1746045X | |
dc.publisher.place | 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND | |
dcterms.isPartOf.abbreviation | Expert. Opin. Drug Discov. | |
crisitem.author.dept | Sonderforschungsbereich 944: Physiologie und Dynamik zellulärer Mikrokompartimente | - |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | organisation19 | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-7839-6397 | - |
crisitem.author.orcid | 0000-0002-2143-2270 | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | YoCh745 | - |
crisitem.author.netid | PiJa938 | - |