An Overexpression Screen in Saccharomyces cerevisiae Identifies Novel Genes that Affect Endocytic Protein Trafficking

DC FieldValueLanguage
dc.contributor.authorArlt, Henning
dc.contributor.authorPerz, Angela
dc.contributor.authorUngermann, Christian
dc.date.accessioned2021-12-23T15:57:55Z-
dc.date.available2021-12-23T15:57:55Z-
dc.date.issued2011
dc.identifier.issn13989219
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/3212-
dc.description.abstractA large number of proteins involved in the biogenesis of yeast endosomes and vacuoles have been identified based on screens that scored for inactivation of proteins. Such screens may, however, miss important regulators of the pathway. Here, we present a visual screen in which we examined the effects on vacuole morphology if any of the 6153 yeast open reading frames was overexpressed. Using a progressive screening procedure, we could identify a total of 53 genes. Among the most striking endosomal proteins are the CORVET/HOPS subunits Vps3, Vps18 and Vps39 and the putative tethering inhibitor Ivy1. Furthermore, six endosomal sorting complex related to transport (ESCRT) proteins led to altered vacuole morphology if overproduced. Among the novel proteins, we identify Yer128w as an endosomal protein that interacts with the AAA-ATPase Vps4, and therefore named it Vfa1 (V ps F our-Associated 1). We present evidence on the possible role of these novel proteins in trafficking to the vacuole. Our data provide novel insights into the regulation of protein trafficking.
dc.description.sponsorshipDFGGerman Research Foundation (DFG)European Commission [SFB 431, 944]; Hans-Muhlenhoff-foundation; We thank Rabea Bartoelke, Franziska Kaplan and Thomas Merkel for their contributions to the screen, Michael Thumm (University of Gottingen, Germany) for the Ape1 antibody and Markus Babst (University of Utah) for plasmids and discussions. This work was supported by the DFG (SFB 431 and 944) and the Hans-Muhlenhoff-foundation (to C. U.).
dc.language.isoen
dc.publisherWILEY-BLACKWELL
dc.relation.ispartofTRAFFIC
dc.subjectCell Biology
dc.subjectCORVET
dc.subjectENDOSOME
dc.subjectESCRT
dc.subjectFUSION
dc.subjectHOMOLOG
dc.subjectHOPS
dc.subjectHOPS TETHERING COMPLEX
dc.subjectMUTANTS
dc.subjectoverexpression screen
dc.subjectPATHWAYS
dc.subjectPHOSPHORYLATION
dc.subjectTRANSPORT
dc.subjectVACUOLAR CARBOXYPEPTIDASE-Y
dc.subjectVps4
dc.subjectYEAST VACUOLE
dc.titleAn Overexpression Screen in Saccharomyces cerevisiae Identifies Novel Genes that Affect Endocytic Protein Trafficking
dc.typejournal article
dc.identifier.doi10.1111/j.1600-0854.2011.01252.x
dc.identifier.isiISI:000295835800011
dc.description.volume12
dc.description.issue11
dc.description.startpage1592
dc.description.endpage1603
dc.contributor.orcid0000-0001-6395-9620
dc.publisher.placeCOMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA
dcterms.isPartOf.abbreviationTraffic
dcterms.oaStatusBronze
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0001-6395-9620-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidArHe830-
crisitem.author.netidUnCh999-
Show simple item record

Page view(s)

3
Last Week
0
Last month
0
checked on Apr 17, 2024

Google ScholarTM

Check

Altmetric