Deep UV Resonance Raman Spectroscopy with a Tunable 4 kHz Nanosecond Solid-State Laser and a 1 mL Circulating Free-Flow System

Autor(en): Broeermann, Andreas
Schluecker, Sebastian
Stichwörter: ACTIVATION; Aromatic Amino Acids; BIOPHYSICAL CHEMISTRY; Chemistry; Chemistry, Physical; Circulating Free-Flow; Deep UV; DYNAMICS; Excitation Profile; EXCITATION PROFILES; NM; PROTEIN SECONDARY STRUCTURE; Resonance Raman Spectroscopy; SPECTROMETER
Erscheinungsdatum: 2011
Herausgeber: OLDENBOURG VERLAG
Journal: ZEITSCHRIFT FUR PHYSIKALISCHE CHEMIE-INTERNATIONAL JOURNAL OF RESEARCH IN PHYSICAL CHEMISTRY & CHEMICAL PHYSICS
Volumen: 225
Ausgabe: 6-7
Startseite: 691
Seitenende: 701
Zusammenfassung: 
Deep UVRR spectra of the aromatic amino acids Phe and Tyr in the wavenumber range 800-1800 cm(-1) with lambda(exc) = 195-208 nm exhibit a selective enhancement of signals arising from vibrations localized in the aromatic ring. For lambda(exc) > 198 nm, the UVRR spectra of Phe and Tyr are dominated by contributions from the in-plane ring stretching modes nu(8a) and nu(8b) at similar to 1600 cm(-1). For lambda(exc) <= 198 nm, intense signals from the symmetric ring stretching, in-plane C-H bending and phenyl-C stretching vibrations below 1400 cm(-1) are observed. Excellent stray light rejection is achieved by a triple monochromator, which can be used either in the additive or subtractive mode for high-resolution and low-wavenumber measurements, respectively. A home-built circulating free-flow system allows the investigation of sample volumes as small as 1 mL.
ISSN: 09429352
DOI: 10.1524/zpch.2011.0078

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