SEQUENCE OF THE GAT OPERON FOR GALACTITOL UTILIZATION FROM A WILD-TYPE STRAIN EC3132 OF ESCHERICHIA-COLI

Autor(en): NOBELMANN, B
LENGELER, JW
Stichwörter: (ESCHERICHIA-COLI); (STRAIN EC3132); Biochemistry & Molecular Biology; Biophysics; DNA SEQUENCE; GALACTITOL; GAT OPERON; GENES; HEXITOL TRANSPORT-SYSTEMS; K-12; PTS
Erscheinungsdatum: 1995
Herausgeber: ELSEVIER SCIENCE BV
Journal: BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
Volumen: 1262
Ausgabe: 1
Startseite: 69
Seitenende: 72
Zusammenfassung: 
The sequence of the gat operon for galactitol (Gat) utilization from a wild-type isolate of Escherichia coli, strain EC3132, is presented. The operon comprises 7 open reading frames (ORFs) called gatYZABCDR. The genes are transcribed from a promoter located upstream of gatY. Genes gatABC encode the substrate-specific domains IIA, IIB and IIC of a galactitol-specific Enzyme II (EII(Gat)) of the phospho enolpyruvate-dependent carbohydrate:phosphotransferase system (PTS); gatD encodes an NAD-dependent Gat 1-phosphate dehydrogenase; and gatY an enzyme which hydrolyses tagatose 1,6-bisphosphate; gene gad is required in a cell to show a Gat(+) phenotype, but its physiological function has not yet been identified; gatR encodes a repressor for the gat operon. All genes are highly similar to the gat genes from E. coli K-12; in this organism they map at 46.70 min of the gene map, equivalent to about 2180-2186 kbp.
ISSN: 01674781
DOI: 10.1016/0167-4781(95)00053-J

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