Fluorescent protein-based reporters reveal stress response of intracellular Salmonella enterica at level of single bacterial cells

DC FieldValueLanguage
dc.contributor.authorSchulte, Marc
dc.contributor.authorOlschewski, Katharina
dc.contributor.authorHensel, Michael
dc.date.accessioned2021-12-23T15:58:18Z-
dc.date.available2021-12-23T15:58:18Z-
dc.date.issued2021
dc.identifier.issn14625814
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/3325-
dc.description.abstractIntracellular bacteria such as Salmonella enterica are confronted with a broad array of defence mechanisms of their mammalian host cells. The ability to sense host cell-imposed damages, and to mount efficient stress responses are crucial for survival and proliferation of intracellular pathogens. The various combinations of host defence mechanisms acting on intracellular bacteria and their individual response also explain the occurrence of distinct subpopulations of intracellular S. enterica such as dormant or persisting, slowly or rapidly replicating cells. Here we describe a set of fluorescence protein (FP)-based reporter strains that were used to monitor the expression of cytoplasmic or periplasmic stress response systems of single bacterial cells. This is mediated by a fast-maturing FP as reporter for induction of stress response genes. We evaluated slower maturing FPs for a second function, that is, the analysis of the status of intracellular proliferation of pathogens. The combination of two FPs allows, at level of single bacterial cells, the interrogation of stress response and intracellular proliferation. Application of these reporters to S. enterica allowed us to detect and quantify distinct intracellular subpopulations with different levels of stress response and proliferation.
dc.description.sponsorshipDeutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [P15, P4]; Hans-MuhlenhoffStiftung; Universitat Osnabruck; Deutsche Forschungsgemeinschaft, Grant/Award Numbers: P15, P4; Hans-MuhlenhoffStiftung, Grant/Award Number: Fellowship to KO; Universitat Osnabruck, Grant/Award Number: profile line P2: Integrated Science
dc.language.isoen
dc.publisherWILEY
dc.relation.ispartofCELLULAR MICROBIOLOGY
dc.subjectantimicrobial activity
dc.subjectCell Biology
dc.subjectcontaining vacuole
dc.subjectcytosolic stress response
dc.subjectEFFECTORS
dc.subjectENDOSOMAL SYSTEM
dc.subjectflow cytometry
dc.subjectfluorescent proteins
dc.subjectIII SECRETION SYSTEM
dc.subjectintracellular pathogen
dc.subjectMicrobiology
dc.subjectPATHOGENICITY ISLAND-2
dc.subjectperiplasmic stress response
dc.subjectPERSISTERS
dc.subjectPROTECTION
dc.subjectREDUCTASE
dc.subjectSalmonella&#8208
dc.subjectstress response
dc.subjectSULFOXIDE
dc.subjectTYPHIMURIUM
dc.subjectVIRULENCE
dc.titleFluorescent protein-based reporters reveal stress response of intracellular Salmonella enterica at level of single bacterial cells
dc.typejournal article
dc.identifier.doi10.1111/cmi.13293
dc.identifier.isiISI:000599346000001
dc.description.volume23
dc.description.issue3
dc.contributor.orcid0000-0001-6604-6253
dc.contributor.orcid0000-0003-4308-2002
dc.contributor.orcid0000-0002-2371-8272
dc.identifier.eissn14625822
dc.publisher.place111 RIVER ST, HOBOKEN 07030-5774, NJ USA
dcterms.isPartOf.abbreviationCell Microbiol.
dcterms.oaStatushybrid, Green Submitted
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0001-6604-6253-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidHeMi480-
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